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Cell death induced by ozone stress in the leaves of Populus deltoides × maximowiczii

  • Published:
Biologia Plantarum

Abstract

When exposed to an acute ozone stress, cell death occurred in leaves of the O3 sensitive Populus deltoides × maximowiczii clone Eridano. After treatment (5 h fumigation and 24 h recovery), the damaged areas covered more than 50 % of the leaf surface. At cellular level, these lesions were preceded by some apoptotic hallmarks and by biochemical and physiological alterations evoked by the apoplastic O3 dissociation. The cell death pattern was highly localized and involved an increase of membrane permeability, externalization of phosphatidylserine, DNA fragmentation, callose accumulation, polyphenol production, and a biphasic oxidative burst accompanied by NO overproduction. These results indicate a process of programmed cell death that could have the biological significance of limiting the spreading the oxidative burst triggered by ozone dissociation in apoplastic environment. Moreover, materials derived from cell dismantling could be remobilized toward developing structures which can conclude their ontogenetic program after the stress.

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Abbreviations

CTAB:

cetyltrimethyl ammonium bromide

DAF-2DA:

4,5-diaminofluorescein diacetate

FITC:

fluoresceine isothiocyanate

H2DCFDA:

2′,7′-dichlorodihydrofluorescein diacetate

HR:

hypersensitive response

MES:

2-(N-morpholino) ethanesulfonic acid

PAL:

phenylalanineammonia lyase

PBS:

phosphate-buffered saline

PCD:

programmed cell death

PI:

propidium iodide

PS:

phosphatidylserine

ROS:

reactive oxygen species

TUNEL:

terminal deoxynucleotidyl transferase

(TdT):

mediated dUTPnick-end labeling

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Authors and Affiliations

  1. Department of Biology, University of Pisa, Pisa, I-56126, Italy

    G. Bartoli, L. M. C. Forino & A. M. Tagliasacchi

  2. Department of Agriculture, Food and Environment, University of Pisa, Pisa, I-56124, Italy

    M. Durante

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  1. G. Bartoli
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  2. L. M. C. Forino
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  3. A. M. Tagliasacchi
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  4. M. Durante
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Correspondence to G. Bartoli.

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Acknowledgements: The authors thank Prof. A. Ranieri and Dr. A. Castagna (Department of Agriculture, Food and Environment, University of Pisa) for ozone fumigation apparatus providing and for the assistance during the fumigation treatment of poplar rooted-cuttings.

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Bartoli, G., Forino, L.M.C., Tagliasacchi, A.M. et al. Cell death induced by ozone stress in the leaves of Populus deltoides × maximowiczii . Biol Plant 57, 514–524 (2013). https://doi.org/10.1007/s10535-013-0315-3

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