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Detection of DNA ladder during cotyledon senescence in cotton

  • Original Papers
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Biologia Plantarum

Abstract

The asynchronous programmed cell death (PCD) in different regions of the cotton cotyledon was studied during senescence. We showed that changes in chlorophyll contents do not occur at the same time in different parts of the cotyledon. By using light microscopy (LM) and the terminal deoxynucleotidyl transferase (TdT)-mediated dUTP nick end in situ labelling (TUNEL) methods, the symptoms of cell death were detected in cotyledon margins earlier than in the central part. DNA ladders were detected by gel electrophoresis in senescent cotyledon margins and in the centre respectively, but not in the whole cotyledons.

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Abbreviations

CTAB:

cetyltrimethyl-ammonium bromide

PCD:

programmed cell death

TE:

tracheary element

TUNEL:

terminal deoxynucleotidyl transferase (TdT)-mediated dUTP nick end in situ labeling

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Authors and Affiliations

  1. State Key Laboratory of Crop Biology, College of Agronomy, Shandong Agricultural University, Tai’an, Shandong, 271018, P.R. China

    Q. E. Xie, I. D. Liu, R. F. Wang, Z. X. Fan, Y. G. Wang & F. F. Shen

  2. Cotton Research Institute, the Chinese Agricultural Academy of Sciences, Anyang, Henan, 455112, P.R. China

    S. X. Yu

Authors
  1. Q. E. Xie
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  2. I. D. Liu
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  3. S. X. Yu
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  4. R. F. Wang
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  5. Z. X. Fan
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  6. Y. G. Wang
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  7. F. F. Shen
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Correspondence to F. F. Shen.

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Xie, Q.E., Liu, I.D., Yu, S.X. et al. Detection of DNA ladder during cotyledon senescence in cotton. Biol Plant 52, 654–659 (2008). https://doi.org/10.1007/s10535-008-0127-z

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  • DOI: https://doi.org/10.1007/s10535-008-0127-z

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