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A type I MADS-box gene is differentially expressed in wheat in response to infection by the stripe rust fungus

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Biologia Plantarum

Abstract

The gene, designated TaMADS2, was obtained from wheat leaves infected with the wheat stripe rust fungus by in silico cloning and RT-PCR. TaMADS2 encodes a predicted 159-amino-acid polypeptide that contains a highly conserved MADS domain. Phylogenetic analysis revealed that TaMADS2 is a type I MADS-box gene. The TaMADS2 transcript was detected in wheat leaves, stems, and roots. The expression of TaMADS2 was substantially down-regulated in the compatible interaction between wheat and Puccinia striiformis f. sp. tritici (Pst) at 36 and 48 h post-inoculation (hpi), whereas in the incompatible interaction the down-regulation was only observed at 48 hpi. Exogenous salicylic acid (SA) and abscisic acid (ABA) greatly induced the expression of TaMADS2 at 12 h post treatment (hpt), whereas methyl jasmonate (MeJA) down-regulated TaMADS2 at 6 hpt by approximately two-fold.

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Abbreviations

hpi:

hour post inoculation

hpt:

hour post treatment

HR:

hypersensitive response

ORF:

open reading frame

qRT-PCR:

quantitative reverse transcription polymerase chain reaction

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Authors and Affiliations

  1. State Key Laboratory of Crop Stress Biology for Arid Areas and College of Plant Protection, Northwest A&F University, Yangling, Shaanxi, 712100, P. R. China

    J. Guo, X. -X. Shi, J. -S. Zhang, P. -F. Bai, X. -N. Guan & Z. -S. Kang

  2. State Key Laboratory of Crop Stress Biology for Arid Areas and College of Life Science, Northwest A&F University, Yangling, Shaanxi, 712100, P. R. China

    Y. -H. Duan

Authors
  1. J. Guo
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  2. X. -X. Shi
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  3. J. -S. Zhang
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  4. Y. -H. Duan
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  5. P. -F. Bai
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  6. X. -N. Guan
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  7. Z. -S. Kang
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Corresponding authors

Correspondence to J. Guo or Z. -S. Kang.

Additional information

Acknowledgments: We thank Prof. L. Dunkle from the USDA-Agricultural Research Service at Purdue University for critically reading the manuscript. This work was supported by National Basic Research Program of China (No. 2013CB127700), National Natural Science Foundation of China (Nos. 30930064 and 31171795), the Fundamental Research Funds for the Central Universities of China (QN2009035) and the 111 Project from Ministry of Education of China (No. B07049).

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Guo, J., Shi, X.X., Zhang, J.S. et al. A type I MADS-box gene is differentially expressed in wheat in response to infection by the stripe rust fungus. Biol Plant 57, 540–546 (2013). https://doi.org/10.1007/s10535-012-0297-6

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  • DOI: https://doi.org/10.1007/s10535-012-0297-6

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