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Synergistic effect of trimethoprim and bavistin for micropropagation of Bacopa monniera

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Biologia Plantarum

Abstract

A micropropagation protocol for Bacopa monniera (L.) Wettst., a medicinally important plant, has been developed. Direct organogenesis without callus formation was induced by culturing node, internode and leaf explants on growth regulator free Murashige and Skoog (MS) medium. MS medium supplemented with an antibiotic trimethoprim (TMP) and a fungicide bavistin (BVN) produced axillary shoots from node and adventitious shoot buds on the surface of all explants. The combination of 200 mg dm−3 TMP and 200 mg dm−3 BVN induced the optimum frequency of shoot formation as well as shoot number. Presence of both TMP and BVN induced multiple axillary shoot formation from the nodal segments and this ability was maintained for four subcultures.

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Abbreviations

BA:

6-benzyladenine

BVN:

bavistin

IAA:

indole-3-acectic acid

IBA:

indole-3-butyric acid

MS:

Murashige and Skoog

TMP:

trimethoprim

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Acknowledgements

J. Singh thanks to UGC, New Delhi for the award of SRF. We are grateful to Dr. A. Singh, Department of Farm Engineering, Institute of Agricultural Sciences, BHU, Varanasi, India, for statistical analysis.

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Authors and Affiliations

  1. Department of Botany, Mahila Mahavidyalaya, Banaras Hindu University, Varanasi, 221005, India

    K. N. Tiwari & J. Singh

  2. Department of Basic Medical Sciences, College of Osteopathic Medicine of The Pacific, Western University of Health Sciences, Pomona, CA, 91766, USA

    V. Tiwari

  3. School of Biotechnology, Faculty of Science, Banaras Hindu University, Varanasi, 221005, India

    B. D. Singh & P. Ahuja

Authors
  1. K. N. Tiwari
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  2. V. Tiwari
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  3. J. Singh
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  4. B. D. Singh
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  5. P. Ahuja
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Correspondence to K. N. Tiwari.

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Tiwari, K.N., Tiwari, V., Singh, J. et al. Synergistic effect of trimethoprim and bavistin for micropropagation of Bacopa monniera . Biol Plant 56, 177–180 (2012). https://doi.org/10.1007/s10535-012-0038-x

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  • DOI: https://doi.org/10.1007/s10535-012-0038-x

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