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Dehydrin and proline content in Brassica napus and B. carinata under cold stress at two irradiances

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Biologia Plantarum

Abstract

The accumulation of cold-induced dehydrin and proline was related to the frost tolerance (FT) in several Brassica species or cultivars. A dehydrin of molecular mass 47 kDa was detected in the leaves of an Ethiopian mustard (B. carinata) and a pair of dehydrins of similar molecular mass in the three (two winter, one spring) oilseed rape (B. napus) cultivars, when plants were maintained at 4 °C for one-month under two different irradiances. More dehydrin was accumulated in oilseed rape than in Ethiopian mustard under the high irradiance. A significant correlation was observed between leaf dehydrin content and FT, and no relationship between proline content and FT or between the proline and dehydrin contents. Protoplast-derived callus cells behaved differently from leaves sampled from intact plants, as they did not accumulate dehydrin and proline in response to cold stress.

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Abbreviations

FT:

frost tolerance

HI:

higher irradiance (400 μmol m−2 s−1)

LI:

lower irradiance (120 μmol m−2 s−1)

LT50 :

lethal temperature

PD:

protoplast-derived

SDS-PAGE:

sodium dodecyl sulfate polyacrylamide gel electrophoresis

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Acknowledgements

This research was supported by the Czech Republic Ministry of Agriculture under projects 0002700604, QH 82285 and QI 111A075. Seed of the three oilseed rape cultivars was generously provided by Oseva Pro Ltd., Opava, Czech Republic, and that of the B. carinata doubled haploid line were developed by the Crop Research Institute, Prague, Czech Republic. We thank K. Kosová, Z. Škodáček, V. Střesková and P. Bartošová for their assistance with plant material and R. Koebner for assistance with the linguistic revision of the manuscript.

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Authors and Affiliations

  1. Crop Research Institute, Drnovská 507, CZ-16106, Prague, Czech Republic

    M. Klíma, P. Vítámvás, M. Vyvadilová & I. T. Prášil

  2. Faculty of Science, Charles University, Viničná 5, CZ-12844, Praque, Czech Republic

    S. Zelenková

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  1. M. Klíma
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  2. P. Vítámvás
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  3. S. Zelenková
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  4. M. Vyvadilová
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  5. I. T. Prášil
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Correspondence to M. Klíma.

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Klíma, M., Vítámvás, P., Zelenková, S. et al. Dehydrin and proline content in Brassica napus and B. carinata under cold stress at two irradiances. Biol Plant 56, 157–161 (2012). https://doi.org/10.1007/s10535-012-0034-1

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  • DOI: https://doi.org/10.1007/s10535-012-0034-1

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