Abstract
Phytohormones are indispensable factors regulating plant cell dedifferentiation. In this paper, different concentrations of 2,4-dichlorophenoxyacetic acid (2,4-D) and kinetin (KIN) were incorporated in the culture medium and the anatomy of dedifferentiated cells prior to callus formation from Arabidopsis thaliana petiole explants was examined. The results indicated that the cytoplasm of parenchyma cells in the vascular bundle gradually became denser with time of culture only if 2,4-D was included in the medium. The WUSCHEL (WUS) gene was expressed in derivative cells of the vascular bundle after culture for 24 h in the presence of 2,4-D and there was no obvious signal in these cells of cultured petioles with KIN alone. These results suggest that 2,4-D plays an important role in the process of dedifferentiation of vascular bundle cells in Arabidopsis petioles and KIN has no obvious effect on it.
Abbreviations
- 2,4-D:
-
2,4-dichlorophenoxyacetic acid
- KIN:
-
kinetin
- WUS:
-
WUSCHEL
References
Atta, R., Laurens, L., Boucheron-Dubuisson, E., Guivarc’h, A., Carnero, E., Giraudat-Pautot, V., Rech, P., Chriqui, D.: Pluripotency of Arabidopsis xylem pericycle underlies shoot regeneration from root and hypocotyl explants grown in vitro. — Plant J. 57: 626–644, 2009.
Brewer, P.B., Heisler, M.G., Hejatko, J., Friml, J., Benkova, E.: In situ hybridization for mRNA detection in Arabidopsis tissue sections. — Nat. Protocols 1:1462-1467, 2006.
Centeno, M.L., Rodríguez, A., Feito, I., Fernández, B.: Relationship between endogenous auxin and cytokinin levels and morphogenic responses in Actinidia deliciosa tissue cultures. — Plant Cell Rep. 16: 58–62, 1996.
Che, P., Lall, S., Nettleton, D., Howell, S.H.: Gene expression programs during shoot, root, and callus development in Arabidopsis tissue culture. — Plant Physiol. 141: 620–637, 2006.
Dello Ioio, R., Linhares, S., Sabatini, S.: Emerging role of cytokinin as a regulator of cellular differentiation. — Curr. Opin. Plant Biol. 11: 23–27, 2008.
Endrizzi, K., Moussian, B., Haecker, A., Levin, J.Z., Laux, T.: The SHOOT MERISTEMLESS gene is required for maintenance of undifferentiated cells in Arabidopsis shoot and floral meristems and acts at a different regulatory level than the meristem genes WUSCHEL and ZWILLE. — Plant J. 6: 967–979, 1996.
Feder, N., Obrien, T.P.: Plant microtechnique: some principles and new methods. — Amer. J. Bot. 55: 123–142, 1968.
Feher, A., Pasternak, T.P., Dudits, D.: Transition of somatic plant cells to an embryogenic state. — Plant Cell Tissue Organ Cult. 74: 201–228, 2003.
Gamborg, O.L., Miller, R.A., Ojima, K.: Nutrient requirements of suspension cultures of soybean root cells. — Exp. Cell Res. 50: 151–158, 1968.
George, L., Eapen, S.: Organogenesis and embryogenesis from diverse explants in pigeonpea (Cajanus cajan L.). — Plant Cell Rep. 13: 417–420, 1994.
Huang, Z.C., Zeng, F.H., Lu, X.Y.: Efficient regeneration of Eucalyptus urophylla from seedling-derived hypocotyls. — Biol. Plant. 54: 131–134, 2010.
Litz, R.E., Hendrix, R.C., Moon, P.A., Chavez, V.M.: Induction of embryogenic mango cultures as affected by genotype, explanting, 2,4-D and embryogenic nurse culture. — Plant Cell Tissue Organ Cult. 53: 13–18, 1998.
Miller, C.O., Skoog, F., Okomura, F.S., Von Saltza, M.H., Strong, F.M.: Isolation, structure and synthesis of kinetin, a substance promoting cell division. — J. amer. chem. Soc. 78: 1375–1380, 1956.
Murashige, T., Skoog, F.: A revised medium for rapid growth and bioassays with tobacco tissue cultures. — Physiol. Plant. 15: 473–497, 1962.
Mitsuoka, T., Honda, H., Xing, X.H., Unno, H.: Effect of intracellular 2,4-D concentration on plantlet regeneration of rice (Oryza sativa L.) callus. — Appl. Microbiol. Biotechnol. 42: 364–366, 1994.
Sandal, I., Kumar, A., Bhattacharya, A., Sharma, M., Shanker, A., Ahuja, P.S.: Gradual depletion of 2,4-D in the culture medium for indirect shoot regeneration from leaf explants of Camellia sinensis (L.) O. Kuntze. — Plant Growth Regul. 47: 121–127, 2005.
Skoog, F., Miller, C.O.: Chemical regulation of growth and organ formation in plant tissue cultured in vitro. — Symp. Soc. exp. Biol. 54: 118–131, 1957.
To, J.P.C., Haberer, G., Ferreira, F.J., Deruere, J., Mason, M.G., Eric Schaller, G., Alonso, J.M., Ecker, J.R., Kieber, J.J.: Type-A Arabidopsis response regulators are partially redundant negative regulators of cytokinin signaling. — Plant Cell 16: 658–671, 2004.
Yeoman, M.M., Evans, P.K.: Growth and differentiation of plant tissue cultures: II. Synchronous cell divisions in developing callus cultures. — Ann. Bot. 31: 323–332, 1967.
Yu, Y., Feng, Z., Wang, G., Li, F., Du, X., Zhu, J.: Initiation of dedifferentiation and structural change in in vitro cultured petiole of Arabidopsis thaliana. — Protoplasma 241: 75–81, 2010.
Yuan, X.J., Wang, Z.Y., Liu, J.X., She, J.M.: Development of a plant regeneration system from seed-derived calluses of centipedegrass [Eremochloa ophiuroides (Munro.) Hack]. — Sci. Hort. 120: 96–100, 2009.
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This work was supported by the National Science Foundation of China (No. 30970169). The first two authors contributed equally to this work.
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Li, F., Cui, X., Feng, Z. et al. The effect of 2,4-D and kinetin on dedifferentiation of petiole cells in Arabidopsis thaliana . Biol Plant 56, 121–125 (2012). https://doi.org/10.1007/s10535-012-0026-1
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DOI: https://doi.org/10.1007/s10535-012-0026-1