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The effects of abiotic stresses on the NADP-dependent malic enzyme in the leaves of the hexaploid wheat

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Biologia Plantarum

Abstract

The objective of this study was to examine the effects of different abiotic stresses on the activity of an NADP-dependent malic enzyme (NADP-ME) and the corresponding gene transcription in the leaves of the hexaploid wheat (Triticum aestivum L.) The activity of the NADP-ME enzyme was increased by water stress (20 % polyethylene glycol 6000), low temperature (4 °C), darkness, salinity (200 mM NaCl), abscisic acid and salicylic acid. The transcription of the TaNADP-ME1 gene decreased in response to all of the stresses except darkness and NaCl. In addition, the transcription of TaNADP-ME2 was down-regulated by all of the tested treatments and could not be detected under dark stress.

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Abbreviations

ABA:

abscisic acid

CAM:

Crassulacean acid metabolism

ME:

malic enzyme

PEG:

polyethylene glycol

SA:

salicylic acid

References

  • Buchanan, B.B.: Role of light in the regulation of chloroplast enzymes. — Annu. Rev. Plant Physiol. 31: 341–374, 1980.

    Article  CAS  Google Scholar 

  • Casati, P., Drincovich, M.F., Edwards, G.E., Andreo, C.S.: Malate metabolism by NADP-malic enzyme in plant defense. — Photosynth Res. 61: 99–105, 1999.

    Article  CAS  Google Scholar 

  • Casati, P., Spampinato, C.P., Andreo, C.S.: Characteristics and physiological function of NADP-malic enzyme from wheat. — Plant Cell Physiol. 38: 928–934, 1997.

    CAS  Google Scholar 

  • Cheng, Y.X., Long, M.: A cytosolic NADP-malic enzyme gene from rice (Oryza sativa L.) confers salt tolerance in transgenic Arabidopsis. — Biotechnol. Lett. 29: 1129–1134, 2007.

    Article  CAS  PubMed  Google Scholar 

  • Chi, W., Yang, J.H., Wu, N.H., Zhang, F.: Four rice genes encoding NADP-malic enzyme exhibit distinct expression profiles. — Biosci. Biotechnol. Biochem. 68: 1865–1874, 2004.

    Article  CAS  PubMed  Google Scholar 

  • Detarsio, E., Maurino, V.G., Alvarez, C.E., Müller, G.L., Andreo, C.S., Drincovich, M.F.: Maize cytosolic NADP-malic enzyme (ZmCytNADP-ME): a phylogenetically distant isoform specifically expressed in embryo and emerging roots. — Plant mol. Biol. 68: 355–367, 2008.

    Article  CAS  PubMed  Google Scholar 

  • Drincovich, M.F., Casati, P., Andreo, C.S.: NADP-malic enzyme from plants: a ubiquitous enzyme involved in different metabolic pathways. — FEBS Lett. 490: 1–6, 2001.

    Article  CAS  PubMed  Google Scholar 

  • Drincovich, M.F., Casati, P., Andreo, C.S., Donahue, R., Edwards, G.E.: UV-B induction of NADP-malic enzyme in etiolated and green maize seedlings. — Plant Cell Environ. 21: 63–70, 1998.

    Article  CAS  Google Scholar 

  • Edwards, G.E., Andreo, C.S.: NADP-malic enzyme from plants. — Phytochemistry 31: 1845–1857, 1992.

    Article  CAS  PubMed  Google Scholar 

  • Edwards, G.E., Nakamoto, H., Brunell, J.N., Hatch, M.D.: Pyruvate Pi dikinase and NADP-malate dehydrogenase in C4 photosynthesis: properties and mechanism of light/dark regulation. — Annu. Rev. Plant Physiol. 36: 255–286, 1985.

    Article  CAS  Google Scholar 

  • Falcone-Ferreyra, M.L., Andreo, C.S., Podestá, F.E.: Purification and physical and kinetic characterization of a photosynthetic NADP-dependent malic enzyme from the CAM plant Aptenia cordifolia. — Plant Sci. 164: 95–102, 2003.

    Article  Google Scholar 

  • Famiani, F., Walker, R.P., László, T., Chen, Z.H., Proietti, P., Leegood, R.C.: An immunohistochemical study of the compartmentation of metabolism during the development of grape (Vitis vinifera L.) berries. — J. exp. Bot. 51: 675–683, 2000.

    Article  CAS  PubMed  Google Scholar 

  • Faske, M., Holtgrefe, S., Ocheretina, O., Meister, M., Backhausen, J.E., Scheibe, R.: Redox equilibria between the regulatory thiols of light/dark-modulated chloroplast enzymes and dithiothretol: finetuning by metabolites. — Biochem. biophys. Acta 1247: 135–142, 1995.

    Article  PubMed  Google Scholar 

  • Ferreira, A.L., Arrabaça, J.D., Vaz-Pinto, V., Lima-Costa, M.E.: Induction of alternative oxidase chain under salt stress conditions. — Biol. Plant. 52: 66–71, 2008.

    Article  CAS  Google Scholar 

  • Fushimi, T., Umeda, M., Shimazaki, T., Kato, A., Toriyama, K., Uchimiya H.: Nucleotide sequence of a rice cDNA similar to a maize NADP-dependent malic enzyme. — Plant mol. Biol. 24: 965–967, 1994.

    Article  CAS  PubMed  Google Scholar 

  • Honda, H., Akagi, H., Shimada, H.: An isozyme of the NADP malic enzyme of a CAM plant, Aloe arborescens, with variation on conservative amino acid residues. — Gene 243: 85–92, 2000.

    Article  CAS  PubMed  Google Scholar 

  • Laporte, M.M., Shen, B., Tarczynski, M.C.: Engineering for drought avoidance: expression of maize NADP-malic enzyme in tobacco results in altered stomatal function. — J. exp. Bot. 53: 699–705, 2002.

    Article  CAS  PubMed  Google Scholar 

  • Liu, S.K., Cheng, Y.X., Zhang, X.X., Guan, Q.J., Shunsaku, N., Kenichi, H., Tetsuo, T.: Expression of an NADP-malic enzyme gene in rice (Oryza sativa. L) is induced by environmental stresses; over-expression of the gene in Arabidopsis confers salt and osmotic stress tolerance. — Plant mol. Biol. 64: 49–58, 2007.

    Article  CAS  PubMed  Google Scholar 

  • Maurino, V.G., Drincovich, M.F., Casati, P., Andreo, C.S., Edwards, G.E., Ku, M.S.B., Gupta, S.K., Franceschi, V.R.: NADP-malic enzyme: immunolocalization in different tissues of the C4 plant maize and the C3 plant wheat. — J. exp. Bot. 48: 799–811, 1997.

    Article  CAS  Google Scholar 

  • Maurino, V.G., Saigo, M., Andreo, C.S., Drincovich, M.F.: Nonphotosynthetic ‘malic enzyme’ from maize: a constituvely expressed enzyme that responds to plant defence inducers. — Plant mol. Biol. 45: 409–420, 2001.

    Article  CAS  PubMed  Google Scholar 

  • Müller, G.L., Drincovich, M.F., Andreo, C.S., Lara, M.V.: Nicotiana tabacum NADP-malic enzyme: cloning, characterization and analysis of biological role. — Plant Cell Physiol. 49: 469–480, 2008.

    Article  PubMed  Google Scholar 

  • Murmu, J., Chinthapalli, B., Raghavendra, A.S.: Light activation of NADP malic enzyme in leaves of maize: marginal increase in activity, but marked change in regulatory properties of enzyme. — J. Plant Physiol. 160: 51–56, 2003.

    Article  CAS  PubMed  Google Scholar 

  • Pandey, D.M., Yeo, U.D.: Stress-induced degradation of D1 protein and its photoprotection by DCPIP in isolated thylakoid membranes of barley leaf. — Biol. Plant. 52: 291–298, 2008.

    Article  CAS  Google Scholar 

  • Pinto, M.E., Casati, P., Hsu, T.P., Ku, M.S.B., Edwards, G.E.: Effects of UV-B radition on growth, photosynthesis, UV-Babsorbing compouns and NADP-malic enzyme in bean (Phaseolus vulgaris L.) grown under different nitrogen conditions. — J. Photochem. Photobiol. B Biol. 48: 200–209, 1999.

    Article  CAS  Google Scholar 

  • Sage, R.F., Seemann, J.R.: Regulation of ribulose-1,5-bisphosphate carboxylase/oxygenase activity in response to reduced light intensity in C4 plants. — Plant Physiol. 102: 21–28, 1993.

    CAS  PubMed  Google Scholar 

  • Saigo, M., Bologna, F.P., Maurino, V.G., Detarsio, E., Andreo, C.S., Drincovich, M.F.: Maize recombinant non-C4 NADP-malic enzyme: a novel dimeric malic enzyme with high specific activity. — Plant mol. Biol. 55: 97–107, 2004.

    Article  CAS  PubMed  Google Scholar 

  • Santos, M.G., Ribeiro, R.V., Machado, E.C., Pimentel, C.: Photosynthetic parameters and leaf water potential of five common bean genotypes under mild water deficit. — Biol. Plant. 53: 229–236, 2009.

    Article  CAS  Google Scholar 

  • Scheibe, R.: NADP+-malate dehydrogenase in C3-plants: regulation and role of a light-activated enzyme. — Physiol. Plant. 71: 393–400, 1987.

    Article  CAS  Google Scholar 

  • Shearer, H.L., Turpin, D.H., Dennis, D.T.: Characterization of NADP-dependent malic enzyme from developing castor oil seed endosperm. — Arch. Biochem. Biophys. 429: 134–144, 2004.

    Article  CAS  PubMed  Google Scholar 

  • Taub, D.R., Lerdau, M.T.: Relationship between leaf nitrogen and photosynthetic rate for three NAD-ME and three NADP-ME in C4 grasses. — Amer. J. Bot. 87: 412–417, 2000.

    Article  Google Scholar 

  • Tausta, S.L., Coyle, H.M., Rothermel, B., Stiefel, V., Nelson, T.: Maize C4 and non-C4 NADP-dependent malic enzymes are encoded by distinct genes derived from a plastidlocalized ancestor. — Plant mol. Biol. 50: 635–652, 2002.

    Article  CAS  PubMed  Google Scholar 

  • Wang, H., Liu, R.L., Jin, J.Y.: Effects of zinc and soil moisture on photosynthetic rate and chlorophyll fluorescence parameters of maize. — Biol. Plant. 53: 191–194, 2009.

    Article  CAS  Google Scholar 

  • Wheeler, M.C.G., Arias, C.L., Tronconi, M.A., Maurino, V.G., Andreo, C.S., Drincovic, M.F.: Arabidopsis thaliana NADP-malic enzyme isoforms: high degree of identity but clearly distinct properties. — Plant mol. Biol. 67: 231–242, 2008.

    Article  PubMed  Google Scholar 

  • Yu, D.J., Kim, S.J., Lee, H.J.: Stomatal and non-stomatal limitations to photosynthesis in field-grown grapevine cultivars. — Biol. Plant. 53: 133–137, 2009.

    Article  CAS  Google Scholar 

Download references

Acknowledgments

This work is part of a doctoral degree program and was supported by grants from National Basic Research Program of China (2010CB951501) and the China National Science and Technology Uphold Plan (2009BADA3B01).

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Authors and Affiliations

  1. Institute of Genetic and Developmental Biology, Chinese Academy of Sciences, Shijiazhuang, 050021, P.R. China

    Z. Y. Fu, Z. B. Zhang, X. J. Hu & P. Xu

  2. Xinjiang Institute of Physics and Chemistry Technology, Chinese Academy of Sciences, Wulumuqi, 830011, P.R.China

    Z. Y. Fu

  3. Graduate University of the Chinese Academy of Sciences, Beijing, 100049, P.R.China

    Z. Y. Fu & X. J. Hu

  4. Institute of Life Sciences, Qingdao University of Science and Technology, Qingdao, 266042, P.R. China

    Z. H. Liu

  5. Experimental and Education Technology Center, Linyi Normal University, Linyi, 276005, P.R.China

    X. J. Hu

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  1. Z. Y. Fu
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  2. Z. B. Zhang
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  3. Z. H. Liu
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  4. X. J. Hu
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  5. P. Xu
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Correspondence to Z. B. Zhang.

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Fu, Z.Y., Zhang, Z.B., Liu, Z.H. et al. The effects of abiotic stresses on the NADP-dependent malic enzyme in the leaves of the hexaploid wheat. Biol Plant 55, 196–200 (2011). https://doi.org/10.1007/s10535-011-0030-x

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  • DOI: https://doi.org/10.1007/s10535-011-0030-x

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