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Protein changes during programmed cell death in tobacco

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  • Published:
Biologia Plantarum

Abstract

Programmed cell death (PCD) was induced by the Yariv reagent in Nicotiana tabacum cv. Bright Yellow-2 cell suspension. The analyses of proteins extracts by 2-D electrophoresis clearly show massive protein degradation which was mainly due to cysteine protease activity. In contrast, some proteins remained unchanged up to 72 h after PCD induction. Peptide mass fingerprints of these proteins, obtained by MALDI-TOF, identified calreticulin, heat shock protein (HSP) 60, HSP70, malate dehydrogenase and mitochondrial ATP synthase β-subunit.

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Abbreviations

AGPs:

arabinogalactan-proteins

ATPase:

ATP synthase β-subunit

BY-2:

Bright Yellow-2

CRT:

calreticulin

2;4-D:

2;4-dichlorophenoxyacetic acid

2-DE:

2-dimensional electrophoresis

IEF:

isoelectric focusing

HSP:

heat shock protein

MALDI-TOF:

matrix assisted laser desorption/ionization-time of fight

MS:

mass spectrometry

MDH:

malatedehydrogenase

MS medium:

Murashige and Skoog medium

PCD:

programmed cell death

TBP:

tributylphosphine

TCA:

trichloroacetic acid

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Acknowledgements

We thank Dr. P. Jackson for the critical reading of the manuscript and Dr. M.J. Kieliszewski for generously providing the BY-2 suspension cultured cells. This research received financial support from Praxis XXI fellowship SFRH/BD/1183/2000.

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Authors and Affiliations

  1. Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa, Av. Republica, EAN, 2780-157, Oeiras, Portugal

    I. Chaves, M. Alves, D. Carrilho, M. C. Duque-Magalhães, C. P. Ricardo & A. P. Regalado

  2. Instituto Superior de Agronomia, Universidade Técnica de Lisboa, Tapada da Ajuda, 1349-017, Lisboa, Portugal

    M. C. Duque-Magalhães & C. P. Ricardo

Authors
  1. I. Chaves
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  2. M. Alves
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  3. D. Carrilho
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  4. M. C. Duque-Magalhães
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  5. C. P. Ricardo
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  6. A. P. Regalado
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Correspondence to C. P. Ricardo.

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Chaves, I., Alves, M., Carrilho, D. et al. Protein changes during programmed cell death in tobacco. Biol Plant 55, 153–158 (2011). https://doi.org/10.1007/s10535-011-0021-y

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