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Sugarcane genes differentially expressed during water deficit

  • Published:
Biologia Plantarum

Abstract

To identify genes that are up and down-regulated by water deficit in sugarcane we used the macroarray methodology and the expression level of 3 575 independent sugarcane cDNAs was measured by hybridization with RNA extracted from plants submitted to mild, moderate and severe water deficit. We identified approximately 1 670 differentially expressed genes from which 62 % were up-regulated by different stress-conditions, whereas many repressed genes were exclusive for each time-point. Analysis of similarity showed that approximately 24 % of the differentially expressed genes shared homology with proteins involved in different processes such as signal transduction, hormone metabolism, photosynthesis, transcription and stress response. Transcripts with no known function accounted for approximately 39 % and those without similarity represented 36 % of the sequences. Five genes analyzed by RT-PCR confirmed the macroarray results.

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Abbreviations

ABA:

abscisic acid

BSA:

bovine serum albumin

bZIP:

basic-leucine zipper

CDPK:

kinase protein calciumdependent

dATG:

deoxyadenosine triphosphate

dCTP:

deoxycytidine triphosphate

dGTP:

deoxyguanosine triphosphate

DTT:

dithiothreitol

dTTP:

deoxythymidine triphosphate

EDTA:

ethylenediaminetetracetic acid

HSP:

heat shock protein

LTP:

lipid transfer proteins

RWC:

relative water content

SAMDC:

s-adenosyl-methionine decarboxylase

SDS:

sodium dodecyl sulfate

SSC:

saline sodium citrate

Tris-HCl:

(hydroxymethyl) aminomethane hydrochloride

ZEP:

zeaxanthin epoxidase

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Acknowledgments

This work was supported by Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP project # 2002/04600-8). F.A. Rodrigues received scholarships from Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) and Coordenação de Aperfeiçoamento de Pessoal de NÍvel Superior (CAPES). We thank MSc. R. de Assis Sordi (Sugarcane Technology Center, Piracicaba-Brazil) for the sugarcane plantlets and Dr. M.C. Roque Barreira (Department of Cellular and Molecular Biology — FMRP-USP) for the use of Phosphorimager FLA3000-G.

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Authors and Affiliations

  1. Brazilian Clone Collection Center, Faculdade de Ciências Agrárias e Veterinárias, UNESP — Univ Estadual Paulista, Jaboticabal, 14884-900, Brazil

    F. A. Rodrigues, J. P. Da Graça & S. M. Zingaretti

  2. Department of Technology, Faculdade de Ciências Agrárias e Veterinárias, UNESP — Univ Estadual Paulista, Jaboticabal, 14884-900, Brazil

    M. L. De Laia, M. I. T. Ferro & J. A. Ferro

  3. Department of Rural Engineering, Faculdade de Ciências Agrárias e Veterinárias, UNESP — Univ Estadual Paulista, Jaboticabal, 14884-900, Brazil

    J. A. Galbiati

  4. Biotechnology Center, Embrapa Trigo, Passo Fundo, RS, 99001-970, Brazil

    A. Nhani-Jr

  5. Department of Florestal Engineering, FCA, UFVJM, Diamantina, MG, 39100-000, Brazil

    M. L. De Laia

  6. Unit of Biotechnology, UNAERP, Ribeirão Preto, SP, 14096-900, Brazil

    S. M. Zingaretti

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  2. J. P. Da Graça
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  3. M. L. De Laia
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  4. A. Nhani-Jr
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  8. S. M. Zingaretti
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Correspondence to S. M. Zingaretti.

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Rodrigues, F.A., Da Graça, J.P., De Laia, M.L. et al. Sugarcane genes differentially expressed during water deficit. Biol Plant 55, 43–53 (2011). https://doi.org/10.1007/s10535-011-0006-x

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