Abstract
Dihydroflavonol 4-reductase (DFR) is a key enzyme in the anthocyanin biosynthesis. In this study, the localization of 45S rDNA and dfr gene (named as CsDFR-bo) of the blood orange (Citrus sinensis Osbeck cv. Ruby) on chromosomes was investigated by fluorescence in situ hybridization (FISH). A karyotype of C. sinensis was reconstructed based on the length of mitotic metaphase chromosomes. The 45S rDNAs were localized on chromosomes 2p and 7q. The detection ratios of 45S rDNA in the two chromosomes were 69.5 and 77.3 %, respectively. The CsDFR-bo was proved to be a single-copy gene and localized on chromosome 3p. The detection ratio of CsDFR-bo was 8.2 %.
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Abbreviations
- DFR:
-
dihydroflavonol 4-reductase
- FISH:
-
fluorescence in situ hybridization
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Part of this work was conducted in the Key Laboratory of MOE for Plant Development Biology in Wuhan University of China.
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Lu, X., Zhou, W. & Gao, F. Chromosomal location of 45S rDNA and dfr gene in Citrus sinensis . Biol Plant 54, 798–800 (2010). https://doi.org/10.1007/s10535-010-0146-4
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DOI: https://doi.org/10.1007/s10535-010-0146-4