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Isolation and expression of gene encoding leucoanthocyanidin reductase from Diospyros kaki during fruit development

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Biologia Plantarum

Abstract

Leucoanthocyanidin reductase (LAR) converts leucoanthocyanidin to (+)-catechin, a precursor of proanthocyanidins abundant in Japanese persimmon (Diospyros kaki Thunb.) fruits. A putative LAR gene (DkLAR) was isolated by rapid amplification of cDNA ends from young fruits. The full-length cDNA of DkLAR gene was 1 356 bp long and encoded an open reading frame of 349 residues. The deduced DkLAR protein was closely related to the homolog in other plant species. The expression of the DkLAR gene in Chinese pollination-constant non-astringent (PCNA) genotype was coincident with the tannin cell development, but was not in Japanese PCNA and Chinese pollination-variant astringent (PCA) genotypes.

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Abbreviations

ANR:

anthocyanidin reductase

CTAB:

cetyl-trimethyl-ammonium bromide

CT:

condensed tannin

IFR:

isoflavone reductase-like proteins

LAR:

leucoanthocyanidin reductase

PA:

proanthocyanidin

PCA:

pollination-constant astringent

PCNA:

pollination-constant non-astringent

PVA:

pollination-variant astringent

PVNA:

pollination variant non-astringent

RACE:

rapid amplification of cDNA ends

RT-PCR:

reverse transcriptase polymerase chain reaction

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Acknowledgements

This research was supported by Natural Science Foundation of China (NSFC No. 30871686 and No. 30921002) and the Research Fund for the Doctoral Program of Higher Education (No. 20070504011).

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Authors and Affiliations

  1. Department of Pomology, Key Laboratory for Horticultural Plant Biology, Huazhong Agricultural University, Wuhan, 430070, P.R. China

    Y. Wang, Q. L. Zhang & Z. R. Luo

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  1. Y. Wang
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  2. Q. L. Zhang
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  3. Z. R. Luo
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Correspondence to Z. R. Luo.

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Wang, Y., Zhang, Q.L. & Luo, Z.R. Isolation and expression of gene encoding leucoanthocyanidin reductase from Diospyros kaki during fruit development. Biol Plant 54, 707–710 (2010). https://doi.org/10.1007/s10535-010-0125-9

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  • DOI: https://doi.org/10.1007/s10535-010-0125-9

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