Abstract
An investigation was undertaken to adjudge the in vitro salt tolerance and biochemical changes due to NaCl on four grape rootstocks (Dogridge, SO4, H-144 and 3309C). The in vitro two-node micro-cuttings from the established cultures were sub-cultured on rooting medium comprising Murashige and Skoog (MS) basal medium supplemented with 0.5 μM indolebutyric acid (IBA), 200 mg dm−3 activated charcoal and different NaCl concentrations ranging from 0 to 125 mM. The surviving and proliferated cultures were further sub-cultured four times on respective media. Dogridge and H-144 tolerated 125 and 100 mM NaCl, respectively, while SO4 and 3309C survived only up to 75 mM NaCl. Contents of proteins, proline, K+ and Na+ in tissue increased in all genotypes due to NaCl supplementation, while contents of chlorophyll and total soluble sugars declined. Higher K+/Na+ ratio was registered in Dogridge and H-144 than in SO4 and 3309C. The relative NaCl tolerance for different grape rootstocks under study could be ranked as Dogridge > H-144 > SO4 and 3309C.
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Abbreviations
- IBA:
-
indolebutyric acid
- MS:
-
Murashige and Skoog medium
References
Amini, F., Ehsanpour, A.A.: Soluble proteins, proline, carbohydrates and Na+/K+ changes in two tomato (Lycopersicon esculentum Mill.) cultivars under in vitro salt stress. — Amer. J. Biochem. Biotechnol. 1: 212–216, 2005.
Ashraf, M., Tufail, M.: Variation in salinity tolerance in sunflower (Helianthus annuus L.). — J. Agron. Crop Sci. 174: 351–362, 1995.
Ashraf, M., Harris, P.J.C.: Potential biochemical indicators of salinity tolerance in plants. — Plant Sci. 166: 3–16, 2004.
Barnes, J.D., Balaguer, L., Manrique, E., Elvira, S., Davison, A.W.: A reappraisal of the use of DMSO for the extraction and determination of chlorophyll ‘a’ and ‘b’ in lichens and higher plants. — Environ. exp. Bot. 32: 85–90, 1992.
Bates, L.S., Waldren, R.D., Teare, I.D.: Rapid determination of free proline for water stress studies. — Plant Soil 39: 205–207, 1973.
Behboudian, M.H., Törökfalvy, E., Walker, R.R.: Effect of salinity on ionic content, water relations and gas exchange parameters in some citrus scion-rootstock combinations. — Scientia Hort. 28: 105–116, 1986.
Bradford, M.M.: A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein dye binding. — Anal. Biochem. 72: 248–254, 1976.
Cavagnaro, J.B., Ponce, M.T., Guzman, J., Cirrincione, M.A.: Argentinean cultivars of Vitis vinifera grow better than European ones when cultured in vitro under salinity. — BioCell 30: 1–7, 2006.
Chandler, S.F., Thorpe, T.A.: Characterization of growth, water relations, and proline accumulation in sodium sulfate tolerant callus of Brassica napus L. cv. Westar (canola). — Plant Physiol. 84: 106–111, 1987.
Erturk, U., Sivritepe, N., Yerlikaya, C., Bor, M., Ozdemir, F., Turkan, I.: Responses of the cherry rootstock to salinity in vitro. — Biol. Plant. 51: 597–600, 2007.
Gorham, J., Wyn Jones, R.G., Bristol, A.: Partial characterization of the trait for the enhanced K+-Na+ discrimination in D genome of wheat. — Planta 180: 590–599, 1990.
Hasegawa, P.M., Bressan, R.A., Zhu, J.K., Bohnert, H.J.: Plant cellular and molecular responses to high salinity. — Ann. Rev. Plant Physiol. mol. Biol. 51: 463–499, 2000.
Jain, R.K., Dhawan, R.S., Sharma, D.R., Chowdhury, J.B.: Salt-tolerance and proline accumulation: A comparative study in salt-tolerant and wild type cultured cells of eggplant. — Plant Cell Rep. 6: 382–384, 1987.
Khawale, R.N., Singh, S.K., Patel, V.B., Singh, S.P.: Changes due to in vitro sodium chloride induced salinity in grape (Vitis vinifera L.). — Indian J. Plant Physiol. (Special issue) 28, 378–382, 2003.
Lin, C.C., Kao, C.H.: Proline accumulation is associated with inhibition of rice seedling root growth caused by NaCl. — Plant Sci. 114: 121–128, 1996.
Moftah, A.E., Michel, B.E.: The effect of sodium chloride on solute potential and proline accumulation in soybean leaves. — Plant Physiol. 83: 238–240, 1987.
Morpurgo, R.: Correlation between potato clones grown in vivo and in vitro under sodium chloride stress conditions. — Plant Breed. 107: 80–82, 1991.
Murashige, T., Skoog, F.: A revised medium for rapid growth and bio-assays with tobacco tissue cultures. — Physiol. Plant. 15: 473–497, 1962.
Nolte, K.D., Hanson, A.D., Gage, D.A.: Proline accumulation and methylation to proline betaine in Citrus: implications for genetic engineering of stress resistance. — J. amer. Soc. hort. Sci. 122: 8–13, 1997.
Pandey, R.M., Divate, M.R.: Salt tolerance in grape I. Effect of sodium salts singly and in combination on some of the morphological characters of grape varieties. — Indian J. Plant Physiol. 19: 230–239, 1976.
Perez-Alfocea, F., Santa-Cruz, A., Guerrier, G., Bolarin, M.C.: NaCl stress-induced organic solute changes on levels and calli of Lycopersicon esculentum, L. pennellii and their inter-specific hybrid. — J. Plant Physiol. 143: 106–111, 1994.
Prior, L.D., Grieve, A.M., Cullis, B.R.: Sodium chloride and soil texture interactions in irrigated field grown sultana grapevines. I. Yield and fruit quality. — Aust. J. agr. Res. 43: 1051–1066, 1992.
Rao, M.S., Jindal, P.C., Dalal, M.A.: In vitro effects of NaCl on leaf damage and chlorophyll content of grapes (Vitis vinifera L.). — Curr. Agr. 15: 35–40, 1991.
Shannon, M.C., Grieve, C.M., Francois, L.E.: Whole-plant response to salinity. — In: Wilkinson, R.E. (ed.): Plant-Environment Interactions. Pp. 199–244. Marcel Dekker, New York 1994.
Singh, A., Saini, M.L. Behl, R.K.: In vitro screening of citrus rootstocks for salt tolerance. — Indian J. Genet. Plant Breed. 64, 54–57, 2004.
Singh, S.K., Khawale, R.N., Singh, S.P.: Technique for rapid in vitro multiplication of Vitis vinifera L. cultivars. — J. hort. Sci. Biotechnol. 19: 267–272, 2004.
Singh, S.K., Sharma, H.C., Goswami, A.M., Dutta, S.P., Singh, S.P.: In vitro growth and leaf composition of grapevine cultivars as affected by sodium chloride. — Biol. Plant. 6: 283–286, 2000.
Sivritepe, N., Eris, A.: Determination of salt tolerance in some grapevine cultivars (Vitis vinifera L.) under in vitro conditions. — Turkish J. Bot. 23: 473–485, 1999.
Sivritepe, N., Erturk, U., Yerlikaya, C., Turkan, I., Bor, M., Ozdemir, F.: Response of the cherry rootstock to water stress induced in vitro. — Biol. Plant. 52: 573–576, 2008.
Stewart, G.R., Lee, J.A.: The role of proline accumulation in halophytes. — Planta 120: 279–289, 1974.
Tewary, P.K., Sharma, A., Raghunath, M.K., Sarkar, A.: In vitro response of promising mulberry (Morus sp.) genotypes for tolerance to salt and osmotic stresses. — Plant Growth Regul. 30: 17–21, 2000.
Thimmaiah, S.R.: Standard Methods of Biochemical Analysis. — Kalyani Publishers, New Delhi 2004.
Toumi, I., Gargouri, M., Nouairi, I., Moschou, P.N., Ben Salem-Fnayou, A., Mliki, A., Zarrouk, M., Ghorbel, A.: Water stress induced changes in the leaf lipid composition of four grapevine genotypes with different drought tolerance. — Biol. Plant. 52: 161–164, 2008.
Vijayan, K., Chakraborti, S.P., Ghosh, P.D.: In vitro screening of mulberry (Morus spp.) for salinity tolerance. — Plant Cell Rep. 22: 350–357, 2003.
Walker, R.B., Douglas, T.J.: Effect of salinity on uptake and distribution of chloride, sodium and potassium ions in citrus plants. — Aust. J. agr. Res. 34: 145–153, 1982.
Acknowledgements
The authors are thankful to Mr. Surender Pal for assistance during in vitro experiments. The first author is thankful to the Ministry of Sciences, Islamic Republic of Iran for granting financial assistance in the form of Overseas Scholarship.
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Alizadeh, M., Singh, S.K., Patel, V.B. et al. In vitro responses of grape rootstocks to NaCl. Biol Plant 54, 381–385 (2010). https://doi.org/10.1007/s10535-010-0069-0
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DOI: https://doi.org/10.1007/s10535-010-0069-0