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In vitro screening of mulberry (Morus spp.) for salinity tolerance

  • Biotic and Abiotic Stress
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Abstract

An efficient in vitro screening method has been developed for mulberry (Morus spp. ) to screen salinity-tolerant genotypes from a large population. Axillary buds from field-grown plants were cultured on MS medium containing five different concentrations (0.0%, 0.25%, 0.5%, 0.75% and 1.00%) of sodium chloride (NaCl) in order to study the shoot growth pattern. Rooting was also tested at four different concentrations of NaCl (0.0%, 0.1%, 0.2% and 0.3.%). NaCl has been found to inhibit the growth and development of mulberry shoots and roots in vitro. The survivability of the axillary buds of the genotypes tested was reduced from 83.7% for the controls to 6.1% in 1.0% NaCl. The average number of roots developed by the genotypes ranged from 11.9 (controls) to 0.2 (0.3% NaCl). Out of the 63 genotypes tested, only seven—Rotundiloba, English black, Kolitha-3, Berhampore-A, Kajli, BC259 and C776—developed roots in 0.3% NaCl. Root growth was also reduced drastically from 1.8 cm for the controls to 0.1 cm in 0.3% NaCl. To test the reproducibility of the results in soil, five tolerant and two susceptible genotypes, identified in this in vitro study, were selected and tested under ex vitro conditions. The significant correlation coefficients obtained between the performances of these genotypes under both types of cultural conditions revealed that in vitro screening of mulberry through axillary bud culture is an easy and efficient method to identify salt-adapted genotypes within a limited space and time period.

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Fig. 1A–F.
Fig. 2a–d.

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Abbreviations

BA :

6-Benzylaminopurine

dS m -1 :

Deci-Siemens per meter

EC :

Electric conductivity

NAA :

α-Naphthaleneacetic acid

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Acknowledgements

The authors express their sincere gratitude to Dr. B. Saratchandra, Director, CSR&TI, Berhampore and Dr. B.N. Roy, Deputy Director, MBG section, CSR&TI, Berhampore for providing the required facilities to carry out the work. Similarly, the help rendered by Dr. K.K. Das, CSR&TI, Berhampore for statistical analysis of the data is gratefully acknowledged.

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Author notes

  1. K. Vijayan

    Present address: SeriBiotech RL, CSB campus, Carmelram (P.O.), 560035, Kodathi, Bangalore, India

  2. P. D. Ghosh

    Present address: Department of Botany, University of Kalyani, 741235, Nadia, West Bengal, India

Authors and Affiliations

  1. Central Sericultural Research and Training Institute, 742 101, Berhampore, West Bengal, India

    K. Vijayan, S. P. Chakraborti & P. D. Ghosh

Authors
  1. K. Vijayan
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  2. S. P. Chakraborti
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  3. P. D. Ghosh
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Correspondence to K. Vijayan.

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Communicated by G.C. Phillips

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Vijayan, K., Chakraborti, S.P. & Ghosh, P.D. In vitro screening of mulberry (Morus spp.) for salinity tolerance. Plant Cell Rep 22, 350–357 (2003). https://doi.org/10.1007/s00299-003-0695-5

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  • DOI: https://doi.org/10.1007/s00299-003-0695-5

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