Skip to main content
SpringerLink
Log in

High efficiency organogenesis and analysis of genetic stability of the regenerants in Solanum melongena

  • Original Papers
  • Published:
Biologia Plantarum

Abstract

A novel protocol for plant regeneration from cotyledon explants of eggplant (Solanum melongena) reducing concentration of sucrose was established. The most efficient bud induction medium consisted of Murashige and Skoog (MS) medium supplemented with 2.0 mg dm−3 zeatin, 0.1 mg dm−3 indoleacetic acid and 10 g dm−3 sucrose. After 15 d, the shoot buds were fragmented and transferred to the shoot elongation MS supplemented with 1.0–2.0 mg dm−3 gibberellic acid and 4.0–8.0 mg dm−3 AgNO3, which promoted shoots elongation. The genetic stability of the regenerated plants was analyzed by flow cytometry, RAPD and SSR molecular markers. The results indicated that almost no somaclonal variation was detected among the regenerants.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

GA3 :

gibberellic acid

IAA:

indoleacetic acid

MS medium:

Murashige and Skoog medium

RAPD:

random amplified polymorphic DNA

SSR:

simple sequence repeat

ZT:

zeatin

References

  • Becher, S.A., Steinmetz, K., Weising, K., Boury, S., Peltier, D., Renou, J.P., Kahl, G., Wolff, K.: Microsatellites for cultivar identification in Pelargonium. — Theor. appl. Genet. 101: 643–651, 2000.

    Article  CAS  Google Scholar 

  • Behera, T.K., Sharma, P., Singh, B.K., Kumar, G., Kumar, R., Mohapatra, T., Singh, N.K.: Assessment of genetic diversity and species relationships in eggplant (Solanum melongena L.) using STMS markers. — Sci. Hort. 107: 352–357, 2006.

    Article  CAS  Google Scholar 

  • Bennett, M.D., Leitch, I.J.: Nuclear DNA amounts in angiosperms. — Ann. Bot. 76: 113–176, 1995.

    Article  CAS  Google Scholar 

  • Borchert, T., Fuchs, J., Winkelmann, T., Hohe, A.: Variable DNA content of Cyclamen persicum regenerated via somatic embryogenesis: rethinking the concept of long-term callus and suspension cultures. — Plant Cell Tissue Organ Cult. 90: 225–263, 2007.

    Article  Google Scholar 

  • Chandrika, M., Thoyajaksha, Ravishankar Rai, V., Ramachandra Kini, K.: Assessment of genetic stability of in vitro grown Dictyospermum ovalifolium. — Biol. Plant. 52: 735–739, 2008.

    Article  CAS  Google Scholar 

  • Ciardi, J., Klee, H.: Regulation of ethylene mediated response at the level of the receptor. — Ann. Bot. 88: 813–822, 2001.

    Article  CAS  Google Scholar 

  • Dayanandan, S., Rajora, O.P., Bawa, K.S.: Isolation and characterization of microsatellites in trembling aspen (Populus tremuloides). — Theor. appl. Genet. 96: 950–956, 1998.

    Article  CAS  Google Scholar 

  • Franklin, G., Sheeba, C.J., Sita, G.L.: Regeneration of eggplant (Solanum melongena L.) from root explant. — In Vitro Cell Dev. Biol. Plant 40: 188–191, 2004.

    Article  Google Scholar 

  • Guo, Y.D., Mattila, Y.T., Pulli, S.: Analysis of timothy (Phleum pratense L.) genetic variation with RAPD and UP-PCR. — Hereditas 138: 101–113, 2003.

    Article  PubMed  Google Scholar 

  • Guo, Y.D., Mizukami, Y., Yamada, T.: Genetic characterization of androgenic progeny derived from Lolium perenne × Festuca pratensis cultivars. — New Phytol. 166: 455–464, 2005.

    Article  CAS  PubMed  Google Scholar 

  • Hyde, C., Phillips, G.C.: Silver nitrate promotes shoot development and plant regeneration of chile pepper (Capsicum annuum L.) via organogenesis. — In Vitro Cell Dev. Biol. Plant 32: 72–80, 1996.

    CAS  Google Scholar 

  • Hussain, Z., Tyagi, R.K., Sharma, R., Agrawal, A.: Genetic diversity in in vitro-conserved germplasm of Curcuma L. as revealed by RAPD markers. — Biol. Plant. 52: 627–633, 2008.

    Article  CAS  Google Scholar 

  • Kamat, M.G., Rao, N.A.: Vegetative multiplication of eggplants (Solanum melongena) using tissue culture techniques. — Plant Sci. Lett. 13: 57–65, 1978.

    Article  CAS  Google Scholar 

  • Kubaláková, M., Doležel, J., Lebeda, A.: Ploidy instability of embryogenic cucumber (Cucumis sativus L.) callus culture. — Biol. Plant. 38: 475–480, 1996.

    Article  Google Scholar 

  • Larkin, P.J., Scowcroft, W.R.: Somaclonal variation — a novel source of variability from cell cultures for plant improvement. — Theor. appl. Genet. 60: 197–214, 1981.

    Article  Google Scholar 

  • Leroy, X.J., Leon, K., Hily, J.M., Chaumeil, P., Branchard, M.: Detection of in vitro culture-induced instability through inter-simple sequence repeat analysis. — Theor. appl. Genet. 102: 885–891, 2001.

    Article  CAS  Google Scholar 

  • Magioli, C., Rocha, A.P.M., De Oliveira, D.E., Mansu, E.: Efficient shoot organogenesis of eggplant (Solanum melongena L.) induced by thidiazuron. — Plant Cell Rep. 17: 661–663, 1998.

    Article  CAS  Google Scholar 

  • Marie, D., Brown, S.C.: A cytometric exercise in plant DNA histograms, with 2C values for 70 species, — Biol. Cell 78: 41–51, 1993.

    Article  CAS  PubMed  Google Scholar 

  • Mohinuddin, A.K.M., Chowdhury, M.K.U., Abdullah Zaliha, C., Napis, S.: Influence of silver nitrate (ethylene inhibitor) on cucumber in vitro shoot regeneration. — Plant Cell Tissue Organ Cult. 51: 75–78, 1997.

    Article  Google Scholar 

  • Mukherjee, S.K., Rathinasabapathi, B., Gupta, N.: Lower sugar and osmotic requirements for shoot regeneration from leaf pieces of Solanum melongena L. — Plant Cell Tissue Organ Cult. 25: 13–16, 1991.

    Article  CAS  Google Scholar 

  • Murashige, T., Skoog, F.: A revised medium for rapid growth and bioassays with tobacco tissue cultures. — Physiol. Plant. 15: 473–497, 1962.

    Article  CAS  Google Scholar 

  • Nassour, M., Chasseriaux, G., Dorion, N.: Optimization of protoplast-to-plant system for Pelargonium × hortorum “Alian” and genetic stability of the regenerated plants. — Plant Sci. 165: 121–128, 2003.

    Article  CAS  Google Scholar 

  • Nunome, T., Suwabe, K., Iketani, H., Hirai M.: Identification and characterisation of microsatellites in eggplant. — Plant Breed. 122: 256–262, 2003.

    Article  CAS  Google Scholar 

  • Phillips, R.L., Kaeppler, S.M., Olhoft, P.: Genetic instability of plant tissue cultures: breakdown of normal controls. — Proc. nat. Acad. Sci. USA 91: 5222–5226, 1994.

    Article  CAS  PubMed  Google Scholar 

  • Picoli, E.A.T., Otoni, W.C., Figueira, M.L., Carolino, S.M.B., Almeida, R.S., Silva, E.A.M., Carvalho, C.R., Fontes, E.P.B.: Hyperhydricity in in vitro eggplant regenerated plants: structural characteristics and involvement of BiP (Binding Protein). — Plant Sci. 160: 857–868, 2001.

    Article  CAS  PubMed  Google Scholar 

  • Qin, Y., Gao, L.H., Pulli, S., Guo, Y.D.: Shoot differentiation, regeneration of cauliflower and analysis of somaclonal variation by RAPD. — Hereditas 143: 91–98, 2006.

    Article  PubMed  Google Scholar 

  • Qin, Y., Li, L.H., Guo, Y.D.: High-frequency embryogenesis, regeneration of broccoli (Brassica oleracea var. italica) and analysis of genetic stability by RAPD. — Sci. Hort. 111: 203–208, 2007.

    Article  CAS  Google Scholar 

  • Rahman, M.H., Dayanandan, S., Rajora, O.P.: Microsatellite DNA markers in Populus tremuloides. — Genome. 43: 293–297, 2000.

    Article  CAS  PubMed  Google Scholar 

  • Sanatombi, K., Sharma, G.J.: In vitro plant regeneration in six cultivars of Capsicum spp. using different explants. — Biol. Plant. 52: 141–145, 2008.

    Article  CAS  Google Scholar 

  • Sharma, P., Rajam, M.V.: Genotype, explant and position effects on organogenesis and somatic embryogenesis in eggplant (Solanum melongena L.). — J. exp. Bot. 46: 135–141, 1995.

    Article  CAS  Google Scholar 

  • Sliwinska, E., Thiem, B.: Genome size stability in six medicinal plant species propagated in vitro. — Biol. Plant. 51: 556–558, 2007.

    Article  Google Scholar 

  • Sreedhar, R.V., Venkatachalam, L., Thimmaraju, R., Bhagyalakshmi, N., Narayan, M.S., Ravishankar, G.A.: Direct organogenesis from leaf explants of Stevia rebaudiana and cultivation in bioreactor. — Biol. Plant. 52: 355–360, 2008.

    Article  CAS  Google Scholar 

  • Yang, X.M., An, L.Z., Xiong, Y.C., Zhang, J.P., Li, Y., Xu, S.J.: Somatic embryogenesis from immature zygotic embryos and monitoring the genetic fidelity of regenerated plants in grapevine. — Biol. Plant. 52: 209–214, 2008.

    Article  CAS  Google Scholar 

  • Yao, H., Zhao, Y., Chen, D.F., Chen, J.K., Zhou, T.S.: ISSR primer screening and preliminary evaluation of genetic diversity in wild populations of Glycyrrhiza uralensis. — Biol. Plant. 52: 117–120, 2008.

    Article  CAS  Google Scholar 

  • Zhang, P., Phansiri, S., Puonti-Kaerlas, J.: Improvement of cassava shoot organogenesis by the use of silver nitrate in vitro. — Plant Cell Tissue Organ Cult. 67: 47–54, 2001.

    Article  CAS  Google Scholar 

Download references

Acknowledgements

This work was partly supported by the grants to Y.-D. Guo (2009CB119000, 2007BAD57B04 and Chinese Universities Scientific Fund 2009-2-06). The first two authors contributed equally to this work.

Author information

Authors and Affiliations

  1. College of Agriculture and Biotechnology, China Agricultural University, 100193, Beijing, P.R. China

    Y. Xing, Y. Yu, X. Luo, B. Zhao & Y. -D. Guo

  2. Institute of Vegetables, Shanxi Academy of Agricultural Sciences, 030031, Taiyuan, P.R. China

    J. -N. Zhang

Authors
  1. Y. Xing
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Y. Yu
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. X. Luo
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. J. -N. Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. B. Zhao
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Y. -D. Guo
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Y. -D. Guo.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Xing, Y., Yu, Y., Luo, X. et al. High efficiency organogenesis and analysis of genetic stability of the regenerants in Solanum melongena . Biol Plant 54, 231–236 (2010). https://doi.org/10.1007/s10535-010-0041-z

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10535-010-0041-z

Additional key words

Search

Navigation