Detection of in vitro culture-induced instability through inter-simple sequence repeat analysis

Abstract 

This paper reports on investigations focused on trinucleotide and tetranucleotide repeats in cauliflower calli (Brassica oleracea var. botrytis L.) and carried out to determine their utility in the detection of genetic variations induced by tissue culture. Out of 224 calli 6 exhibited original patterns; in one of these, PCR patterns differed at four polymorphic loci. The observed tetranucleotide-repeat classes were polymorphic, whereas fingerprinting patterns were stable with (CAG)₅. The most frequent polymorphic and useful primer for detecting genetic variation appeared to be (CAA)₅. We also characterised an Inter-Simple Sequence Repeat (ISSR) marker homologous to a gene involved in cellular proliferation, and modifications of this gene on callogenesis and/or differentiation are examined.