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Nitric oxide alleviates Fe deficiency-induced stress in Solanum nigrum

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Biologia Plantarum

Abstract

The possible involvement of nitric oxide in response of Solanum nigrum seedlings to Fe deficiency was investigated. Iron deficiency resulted in decreased shoot height and chlorophyll content and increased proliferation of root hairs and H2O2, K+ and Ca2+ content. NO donor S-nitrosoglutathione (GSNO) was effective in preventing Fe deficiency-induced increase in content of H2O2 and the ion uptake. The protective effects of GSNO were reversed by cPTIO, an NO scavenger, and tungstate, a nitrate reductase (NR) inhibitor.

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Abbreviations

cPTIO:

2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide

DAB:

3-diaminobenzidine

DAF-2 DA:

4,5-diaminofluorescein diacetate

GSNO:

S-nitrosoglutathione

L-NAME:

NG-nitro-L-Arg-methyl ester

NR:

nitrate reductase

NOS:

nitric oxide synthase

ROS:

reactive oxygen species

TCA:

trichloroacetic acid

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Authors and Affiliations

  1. Center for Agricultural Resources Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Huaizhong RD 286, Shijiazhuang, 050021, P.R. China

    J. Xu, X. J. Liu, T. Yuan, L. L. Yang, Z. X. Xie & W. Y. Wang

  2. Center for Agricultural Resources Research, Institute of Genetics and Developmental Biology, College of Life Science and Geography, Qinghai Normal University, Wusi RD 38, Xining, 810008, P.R. China

    H. X. Yin & Q. Mi

Authors
  1. J. Xu
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  2. H. X. Yin
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  3. X. J. Liu
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  4. T. Yuan
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  5. Q. Mi
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  6. L. L. Yang
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  7. Z. X. Xie
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  8. W. Y. Wang
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Corresponding authors

Correspondence to J. Xu or W. Y. Wang.

Additional information

Acknowledgements: We thank Dr. Dezhu Li of Kunming Institute of Botany, Chinese Academy of Sciences for providing the S. nigrum seeds. The authors truly appreciate the time that Dr. Jana Pospíšilová and the anonymous reviewers spent on helping to clarify the confusions and modify the paper. The research was supported by the Knowledge Innovation Program of the Chinese Academy of Sciences (Grant no. 0707013603)) and the National Major Special Project on New Varieties Cultivation for Transgenic Organisms (Grant no. 2009ZX08009-130B).

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Xu, J., Yin, H.X., Liu, X.J. et al. Nitric oxide alleviates Fe deficiency-induced stress in Solanum nigrum . Biol Plant 53, 784–788 (2009). https://doi.org/10.1007/s10535-009-0144-6

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  • DOI: https://doi.org/10.1007/s10535-009-0144-6

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