Abstract
This report describes an efficient plant regeneration system for the medicinal plant Lythrum salicaria via direct adventitious shoot development from leaf and stem explants. Leaf explants were much more responsive to regeneration than stem segments. Of the hormonal combinations tested, those involving thidiazuron (TDZ; 0.1, 0.3 or 0.5 mg dm−3) were more effective than the combinations of other hormones and 0.1 mg dm-3 TDZ combined with either indole-3-acetic acid (IAA) or indole-3-butyric acid (IBA) was the most productive. Rooting was readily achieved when multiple shoots were singled out and cultured on medium containing different auxins. IAA was the most effective on root development in terms of both the number of roots per shoot and the frequency of rooted shoots. More than 90 % of the regenerants survived after hardening for four weeks at gradually decreased air humidity.
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Abbreviations
- BA:
-
benzyladenine
- 2,4-D:
-
2,4-dichlorophenoxyacetic acid
- GA3 :
-
gibberellin
- IAA:
-
indole-3-acetic acid
- IBA:
-
indole-3-butyric acid
- KIN:
-
kinetin
- MSMO:
-
Murashige and Skoog medium with minimum organics
- NAA:
-
naphthaleneacetic acid
- TDZ:
-
thidiazuron
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Acknowledgements: The authors are grateful to The Scientific and Technological Research Council of Turkey (TUBITAK) for financial support [TBAG-2278(103T024) and TBAG-HD/27(105T040)]. Special thanks are expressed to Dr. Hakan Turker for his technical support and Dr. Kemal Kazan (CSIRO Plant Industry, Australia) for the critical reading of the manuscript.
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Turker, A.U., Yucesan, B. & Gurel, E. An efficient in vitro regeneration system for Lythrum salicaria . Biol Plant 53, 750–754 (2009). https://doi.org/10.1007/s10535-009-0137-5
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DOI: https://doi.org/10.1007/s10535-009-0137-5