Abstract
A simple and reliable regeneration system from leaf bases of barley (Hordeum vulgare L.) has been developed. The in vitro regeneration frequencies of seven commercial barley genotypes were compared using segments from the first leaves of 5-d-old seedlings. The regeneration frequency ranged from 31.56 to 72.22 % among the barley genotypes. Murashige and Skoog medium supplemented with 6-benzyladenine (0.5 mg dm−3) and kinetin (0.5 mg dm−3) was optimum for the regeneration. Longitudinal cut of the segments or the removal of coleoptiles further increased plantlet regeneration frequency.
This is a preview of subscription content, log in via an institution to check access.
Abbreviations
- ABA:
-
abscisic acid
- KIN:
-
kinetin
- BA:
-
6-benzyladenine
- MS:
-
Murashige and Skoog
- 2,4-D:
-
dichlorophenoxyacetic acid
References
Chung, H.H., Chen, J.T., Chang, W.C.: Plant regeneration through direct somatic embryogenesis from leaf explants of Dendrobium. — Biol. Plant. 51: 346–350, 2007.
Dahleen, L.S., Bregitzer, P.: An improved media system for high regeneration rates from barley immature embryo-derived callus cultures of commercial cultivars. — Crop Sci. 42:934–938, 2002.
Gless, C., Lörz, H., Jähne-Gärtner, A.: Transgenic oat plants obtained at high efficiency by microprojectile bombardment of leaf base segments. — J. Plant Physiol. 152: 151–157, 1998a.
Gless, C., Lörz, H., Jähne-Gärtner, A.: Establishment of a highly efficient regeneration system from leaf base segments of oat (Avena sativa L.). — Plant Cell Rep. 17: 441–445, 1998b.
Haliloglu, K.: Efficient regeneration system from wheat leaf base segments. — Biol. Plant. 50: 326–330, 2006.
Maheswari, M., Lakshmi, J.N., Yadav, S.K., Varalaxmi, Y., Lakshmi, A.V., Vanaja, M., Venkateswarlu, B.: Efficient plant regeneration from shoot apices of sorghum. — Biol. Plant. 50: 741–744, 2006.
Mingozzi, M., Morini, S.: In vitro cultivation of donor quince shoots affects subsequent morphogenesis in leaf explants. — Biol. Plant. 53: 141–144, 2009.
Murashige, T., Skoog, F.: A revised medium for rapid growth and bioassays with tobacco tissue cultures. — Physiol. Plant. 15: 473–497, 1962.
Orban, N., Boldizsar, I., Boka, K.: Structural and chemical study of callus formation from leaves of Rubia tinctorum. — Biol. Plant. 51: 421–429, 2007.
Pasternak, T.P., Rudas, V.A., Lörz, H., Kumlehn, J.: Embryogenic callus formation and plant regeneration from leaf base segments of barley (Hordeum vulgare L.). — J. Plant Physiol. 155: 371–375, 1999.
Rengel, Z., Jelaska, S.: Somatic embryogenesis and plant regeneration from seedling tissues of Hordeum vulgare L. — Plant Physiol. 124: 385–392, 1986.
Sujatha, M., Kumar, D.V.: In vitro bud regeneration of Carthamus tinctorius and wild Carthamus species from leaf explants and axillary buds. — Biol. Plant. 51: 782–786, 2007.
Wang, C.T., Wei, Z.M.: Embryogenesis and regeneration of green plantlets from wheat (Triticum aestivum) leaf base. — Plant Cell Tissue Organ Cult. 77: 149–156, 2004.
Author information
Authors and Affiliations
Corresponding author
Additional information
Acknowledgements: This work was supported by the National Natural Science Foundation of China (30530510), the Ministry of Science and Technology of China (2007AA100505) and the Doctoral Program of Higher Education of China (20070504010).
Rights and permissions
About this article
Cite this article
Li, H.P., Huang, T., Wang, C.X. et al. An efficient regeneration system of barley cultivars from leaf base segments. Biol Plant 53, 733–736 (2009). https://doi.org/10.1007/s10535-009-0133-9
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10535-009-0133-9