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The effect of yeast extract and methyl jasmonate on rosmarinic acid accumulation in Coleus blumei hairy roots

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Biologia Plantarum

Abstract

The leaves of axenically grown Coleus blumei were inoculated with Agrobacterium rhizogenes strain A4 and hairy root were established. PCR and Southern hybridization analysis confirmed transgenic nature of hairy root clones. Cultures of normal roots, induced by α-naphthaleneacetic acid on leaf explants, and hairy roots were evaluated for growth and rosmarinic acid content. Significantly better growth and up to 2.8 higher amount of rosmarinic acid was detected by HPLC analysis in hairy root clones. Methyl jasmonate stimulated rosmarinic acid accumulation in 6 out of 11 tested clones, while yeast extract induced RA accumulation in two and diminished it in 5 out of 11 tested hairy root clones.

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Abbreviations

CB:

medium proposed for Coleus blumei (Petersen and Alfermann 1988)

FM:

fresh mass

MJ:

methyl jasmonate

MS:

Murashige and Skoog medium (1962)

NAA:

α-naphthaleneacetic acid

NR:

normal, non-transformed roots

PAL:

phenylalanine ammonia lyase

RA:

rosmarinic acid

YE:

yeast extract

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Authors and Affiliations

  1. Department of Molecular Biology, Faculty of Science, University of Zagreb, Horvatovac 102a, 10000, Zagreb, Croatia

    N. Bauer, D. Kiseljak & S. Jelaska

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  1. N. Bauer
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  2. D. Kiseljak
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  3. S. Jelaska
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Correspondence to N. Bauer.

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Acknowledgements: Research was supported by the Ministry of Science of the Republic of Croatia within the framework of the project: 119-1191196-1225 “Factors and markers for plant embryogenic potential and phenolic metabolism”. We thank Dr. Mirta Tkalec for statistical analysis of data.

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Bauer, N., Kiseljak, D. & Jelaska, S. The effect of yeast extract and methyl jasmonate on rosmarinic acid accumulation in Coleus blumei hairy roots. Biol Plant 53, 650–656 (2009). https://doi.org/10.1007/s10535-009-0118-8

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  • DOI: https://doi.org/10.1007/s10535-009-0118-8

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