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Somatic embryogenesis in Araucaria angustifolia

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Biologia Plantarum

Abstract

Immature and mature zygotic embryos were used as source of explants for induction of somatic embryogenesis in Araucaria angustifolia. Embryogenic cultures (EC) were only obtained from immature zygotic embryos. Basic medium, carbon source, and genotype showed a significant influence on the formation of stage I somatic embryos (SE). When EC were submitted to maturation conditions, SE continued their individual development until stage II, but mature embryos were not obtained. Proteins secreted by embryogenic cultures were, to a certain degree, genotype specific and included an extracellular class IV chitinase and β-1-3-glucanase.

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Abbreviations

ABA:

abscisic acid

BA:

6-benzylaminopurine

2,4-D:

2,4-dichlorophenoxyacetic acid

Kin:

kinetin

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Authors and Affiliations

  1. Institute of Plant Biochemistry and Biotechnology, University of Muenster, D-48149, Muenster, Germany

    A. L. Wendt dos Santos & B. M. Moerschbacher

  2. Department of Plant Science, Federal University of Santa Catarina, 88034-001, Florianópolis, Brazil

    N. Steiner & M. P. Guerra

  3. Institute of Biology, Humboldt University, D-10115, Berlin, Germany

    K. Zoglauer

Authors
  1. A. L. Wendt dos Santos
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  2. N. Steiner
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  3. M. P. Guerra
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  4. K. Zoglauer
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  5. B. M. Moerschbacher
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Correspondence to B. M. Moerschbacher.

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Wendt dos Santos, A.L., Steiner, N., Guerra, M.P. et al. Somatic embryogenesis in Araucaria angustifolia . Biol Plant 52, 195–199 (2008). https://doi.org/10.1007/s10535-008-0044-1

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  • DOI: https://doi.org/10.1007/s10535-008-0044-1

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