Skip to main content
SpringerLink
Log in

Comparison of induction frequency, maturation capacity and germination of Abies numidica during secondary somatic embryogenesis

  • Brief Communication
  • Published:
Biologia Plantarum

Abstract

Efficiency of the method for improving repetitive somatic embryogenesis and plant recovery of Algerian fir (Abies numidica De Lann.) was investigated by evaluating of induction frequency, maturation capacity and germination. Individual zygotic embryos differed only slightly in induction frequencies (6.8 %) from somatic embryos of the first (5.7 %) and second cycle (5.5–9.0 %). The yield of mature embryos differed significantly among the cell lines of the same cycle and among cell lines of the different cycles. Percentage of abnormalities was lowest in the first cycle of somatic embryos, whereas the second and the third cycles of somatic embryos were branded by a higher frequency of abnormalities. The differences in germination of well developed somatic embryos depended on cell lines rather than on cycle of somatic embryos.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Abbreviations

ABA:

abscisic acid

BAP:

benzylaminopurine

GL:

L-glutamine

CH:

casein hydrolysate

ESM:

embryonal suspensor mass

MS:

Murashige and Skoog (1962) medium

PEG-4000:

polyethylene glycol-4000

SH:

Schenk and Hildebrandt (1972) medium

References

  • Eastman, P.A., Webster, F.B., Pitel, J.A., Roberts, D.R.: Evaluation of somaclonal variation during somatic embryogenesis of interior spruce (Picea glauca engelmannii complex) using culture morphology and isozyme analysis.-Plant Cell Rep. 10: 425–430, 1991.

    Article  Google Scholar 

  • Griga, M.: Morphology and anatomy of Pisum sativum somatic embryos.-Biol. Plant. 45: 173–182, 2002.

    Article  Google Scholar 

  • Jalonen, P., Von Arnold, S.: Characterization of embryogenic cell lines of Picea abies in relation to their competence for maturation.-Plant Cell Rep. 10: 384–387, 1991.

    Article  Google Scholar 

  • Kormuťák, A., Vooková, B.: Somatic embryogenesis and genetic stability of embling in Algerian fir (Abies numidica De Lann.).-In: Iljev, I., Zhelev, P., Tzvetkov, I., Gjuleva, V., Smidt, G. (ed.): IPPS in Bulgaria. Pp. 65–69. Forest University, Sofia 2000.

    Google Scholar 

  • Lelu, M.K., Klimaszewska, K., Charest, P.J: Somatic embryogenesis from immature and mature zygotic embryos and from cotyledons and needles of somatic plantlets of Larix.-Can. J. Bot. 24: 100–106, 1994.

    Google Scholar 

  • Mo, L.H., Egertsdotter, U., Von Arnold, S.: Secretion of specific extracellular proteins by somatic embryos of Picea abies is depended on embryo morphology.-Ann. Bot. 77: 143–152, 1996.

    Article  CAS  Google Scholar 

  • Murashige, T., Skoog, F.: A revised medium for rapid growth and bioassays with tobaco tissue cultures.-Physiol. Plant. 15: 473–497, 1962.

    Article  CAS  Google Scholar 

  • Nørgaard, J.V., Krogstrup, P.: Somatic embryogenesis in Abies spp. In: Jain, S.M., Gupta, P.K., Newton, R.J. (ed.): Somatic Embryogenesis in Woody Plants. Vol. 3. Pp. 341–355. Kluwer Academic Publishers, Dordrecht 1995.

    Google Scholar 

  • Raemarkers, C.J., Jacobsen, E., Visser, R.G.F.: Secondary somatic embryogenesis and application in plant breeding.-Euphytica 81: 93–107, 1995.

    Article  Google Scholar 

  • Salaj, T., Salaj, J.: Somatic embryo formation on mature Abies alba × Abies cephalonica zygotic embryo explants.-Biol. Plant. 47: 7–11, 2003/04.

    Article  CAS  Google Scholar 

  • Saly, S., Joseph, C., Corbineau, F., Lelu, M.A., Côme, D.: Induction of secondary somatic embryogenesis in hybrid larch (Larix × leptoeuropaea) as related to ethylene.-Plant Growth Regul. 37: 287–294, 2002.

    Article  CAS  Google Scholar 

  • Schenk, R.V., Hildebrandt, A. C.: Medium and techniques for induction and growth of monocotyledonous plant cell cultures.-Can. J. Bot. 50: 199–204, 1972.

    CAS  Google Scholar 

  • Vooková, B., Gajdošová, A., Matúšová, R.: Somatic embryogenesis in Abies alba × Abies alba and Abies alba × Abies nordmanniana hybrids.-Biol. Plant. 40: 523–531, 1997/98.

    Article  Google Scholar 

  • Vooková, B., Kormuťák, A.: Effect of sucrose concentration, charcoal, and indole-3-butyric acid on germination of Abies numidica somatic embryos.-Biol. Plant. 44: 181–184, 2001.

    Article  Google Scholar 

  • Vooková, B., Matúšová, R., Kormuťák, A.: Secondary somatic embryogenesis in Abies numidica De Lann.-Biol. Plant. 46: 513–517, 2003

    Article  Google Scholar 

  • Wann, S.R., Johnson, M.A. Noland, T.L., Carlson, J.A.: Biochemical differences between embryogenic and nonembryogenic callus of Picea abies (L) Karst.-Plant Cell Rep. 6: 39–42, 1987.

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Institute of Plant Genetics and Biotechnology, Slovak Academy of Science, Akademická 2, P.O. Box 39A, SK-95007, Nitra, Slovak Republic

    B. Vooková & A. Kormuťák

Authors
  1. B. Vooková
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. A. Kormuťák
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to B. Vooková.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Vooková, B., Kormuťák, A. Comparison of induction frequency, maturation capacity and germination of Abies numidica during secondary somatic embryogenesis. Biol Plant 50, 785–788 (2006). https://doi.org/10.1007/s10535-006-0132-z

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10535-006-0132-z

Additional key words

Search

Navigation