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Limited variability of CTG/CAG repeats in Lycopersicon nuclear DNA

  • Brief Communication
  • Published:
Biologia Plantarum

Abstract

Seven clones containing (CTG)n/(CAG)n repeats (n ≥ 4) were isolated by screening Lycopersicon esculentum genomic DNA. Four of the clones contained more than one simple sequence repeat (SSR). The SSRs were analyzed in several L. esculentum cultivars after polymerase chain reaction (PCR) amplification. No length variations were observed, suggesting considerable locus stability. Five clones are from transcribed regions, which might explain the lack of cultivar variations. However the conservation of CTG repeats was limited as differences in some transcribed loci were registered between L. pennellii and other Lycopersicon species. It is noted that in Lycopersicon trinucleotide repeat variation might be used for species identification.

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Abbreviations

EST:

expressed sequence tags

PCR:

polymerase chain reaction

SDS:

sodium dodecyl sulfate

1× SSC:

0.15 M sodium chloride/0.015 M sodium citrate, pH 7.0

SSR:

simple sequence repeat

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Authors and Affiliations

  1. Institute of Biophysics, Bulgarian Academy of Sciences, Acad. G. Bonchev Str. Bl. 21, 1113, Sofia, Bulgaria

    D. Dimitrova

  2. Institut für Molekularbiologie II, Universität Zurich, Winterthurer Str. 190, CH-8057, Zurich, Switzerland

    O. Georgiev

  3. Fritz Liepmann Institut für Altersforschung, Beutenberger Str. 11, D-07745, Jena, Germany

    C. Valkova

  4. Institute of Genetics, Bulgarian Academy of Sciences, 1113, Sofia, Bulgaria

    B. Atanassova

  5. Friedrich-Schiller Universität Jena, Drackendörfer Str. 1, D-07747, Jena, Germany

    L. Karagyozov

Authors
  1. D. Dimitrova
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  2. O. Georgiev
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  3. C. Valkova
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  4. B. Atanassova
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  5. L. Karagyozov
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Correspondence to B. Atanassova.

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Dimitrova, D., Georgiev, O., Valkova, C. et al. Limited variability of CTG/CAG repeats in Lycopersicon nuclear DNA. Biol Plant 52, 149–152 (2008). https://doi.org/10.1007/s10535-008-0031-6

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  • DOI: https://doi.org/10.1007/s10535-008-0031-6

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