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In vitro plant regeneration in six cultivars of Capsicum spp. using different explants

  • Brief Communication
  • Published:
Biologia Plantarum

Abstract

In vitro regeneration from leaf, cotyledon and hypocotyl explants of six cultivars belonging to three species of Capsicum was achieved by direct organogenesis. The cultivar Umorok showed the best response while Meiteimorok, Haomorok, Mashingkha and Uchithi showed intermediate response and the cultivar Chiengpi was the least responsive. Leaf and cotyledon explants regenerated more shoots than hypocotyl explants and the maximum number of shoots were produced on Murashige and Skoog (1962) medium containing 8.8 µM 6-benzylaminopurine (BAP) with 11.4 µM indole-3-acetic acid (IAA). Elongation of shoot buds derived from different explants was achieved on medium containing 2.8 µM IAA and the elongated shoots were rooted on medium containing 2.8 or 5.7 µM IAA and 2.4 or 4.9 µM indole-3-butyric acid (IBA). Four-week old rooted plantlets were hardened and transplanted to the soil. The plantlets showed 90 % survival during transplantation.

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Abbreviations

BAP:

6-benzylaminopurine

IAA:

indole-3-acetic acid

IBA:

indole-3-butyric acid

MS:

Murashige and Skoog

NAA:

α-naphthalene acetic acid

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Authors and Affiliations

  1. Department of Life Sciences, Manipur University, Imphal, 795003, India

    K. Sanatombi & G. J. Sharma

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  1. K. Sanatombi
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  2. G. J. Sharma
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Correspondence to G. J. Sharma.

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Sanatombi, K., Sharma, G.J. In vitro plant regeneration in six cultivars of Capsicum spp. using different explants. Biol Plant 52, 141–145 (2008). https://doi.org/10.1007/s10535-008-0029-0

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  • DOI: https://doi.org/10.1007/s10535-008-0029-0

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