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Endogenous abscisic acid and protein contents during seed development of Araucaria angustifolia

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Biologia Plantarum

Abstract

This paper describes a proteome analysis and changes in endogenous abscisic acid (ABA) contents during seed development of Araucaria angustifolia (Bert.) O. Ktze. Megagametophytes and embryonic axis tissues exhibited a similar ABA variation pattern during seed development, reaching maximum values at the pre-cotyledonary stage. The embryonic axis protein content increased until the cotyledonary stage with following stabilization at mature seed. The two-dimensional electrophoresis at the torpedo developmental stage showed approximately 230 polypeptides against 340 in the mature stage. Peptide mass fingerprinting analyses identified three polypeptides, corresponding to an AtSAC4, a late embryogenesis abundant (LEA) and a storage protein, respectively.

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Abbreviations

ABA:

abscisic acid

f.m.:

fresh matter

HPLC:

high performance liquid chromatography

LEA:

late embryogenesis abundant protein

PMF:

peptide mass fingerprinting

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Authors and Affiliations

  1. Biotechnology Laboratory, CBB-UENF, 28.013-602, Campos dos Goytacazes-Rio de Janeiro, Brazil

    V. Silveira

  2. Department of Botany, IB-University of São Paulo, CP 11461, 05422-970, São Paulo-SP, Brazil

    C. Santa-Catarina, T. S. Balbuena, W. Handro & E. I. S. Floh

  3. Department of Cell Biology, IB-University of Brasilia, 70910-900, Brasília-DF, Brazil

    F. M. S. Moraes, C. A. O. Ricart & M. V. Sousa

  4. Department of Plant Science, CCA-Federal University of Santa Catarina, 88034-001, Florianópolis-SC, Brazil

    M. P. Guerra

Authors
  1. V. Silveira
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  2. C. Santa-Catarina
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  3. T. S. Balbuena
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  4. F. M. S. Moraes
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  5. C. A. O. Ricart
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  6. M. V. Sousa
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  7. M. P. Guerra
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  8. W. Handro
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  9. E. I. S. Floh
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Corresponding author

Correspondence to E. I. S. Floh.

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Silveira, V., Santa-Catarina, C., Balbuena, T.S. et al. Endogenous abscisic acid and protein contents during seed development of Araucaria angustifolia . Biol Plant 52, 101–104 (2008). https://doi.org/10.1007/s10535-008-0018-3

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  • DOI: https://doi.org/10.1007/s10535-008-0018-3

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