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Evaluation of heterologous promoters in transgenic Populus tremula × P. alba plants

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Biologia Plantarum

Abstract

The pattern and expression level of β-glucuronidase (gus) reporter gene regulated by six heterologous promoters were studied in transgenic Populus tremula × P. alba plants obtained by Agrobacterium-mediated transformation. Binary vector constructs used contained the following promoter sequences: the CaMV35S from cauliflower mosaic virus; its duplicated version fused to the enhancer sequence from alfalfa mosaic virus; CsVMV from cassava vein mosaic virus; ubiquitin 3 from Arabidopsis thaliana (UBQ3); S-adenosyl-L-methionine synthetase (Sam-s) from soybean; and the rolA from Agrobacterium rhizogenes. Histochemical staining of root, stem and leaf tissues showed phloem and xylem-specific gus expression under rolA promoter, and constitutive expression with the other putative constitutive promoters. Quantitative GUS expression of 10 – 15 independently transformed in vitro grown plants, containing each promoter, was determined by fluorimetric GUS assays. The UBQ3-gus fusion induced the highest average expression level, although an extensive variation in expression levels was observed between independent transgenic lines for all the constructs tested.

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Abbreviations

35S-D:

duplicated version of CaMV35S promoter fused to the enhancer sequence from alfalfa mosaic virus

CaMV35S:

35S promoter from cauliflower mosaic virus

CsVMV:

promoter from cassava vein mosaic virus

EDTA:

ethylene diamine tetraacetic acid

GUS:

β-glucuronidase

MU:

4-methylumbelliferone

MUG:

4-methylumbelliferyl-β-D-glucuronide

rolA:

promoter from rolA gene from Agrobacterium rhizogenes

Sam-s:

promoter from S-adenosyl-L-methionine synthetase gene from Glycine max

UBQ3:

promoter from ubiquitin 3 gene from Arabidopsis thaliana

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Author notes

  1. A. C. M. Brasileiro

    Present address: Embrapa Labex-France, Cirad-Amis, TA 40/03, Avenue Agropolis, 34.398, Montpellier Cedex 5, France

  2. C. Studart-Guimaraes

    Present address: Max-Planck-Institute of Molecular Plant Physiology, Am Muhlenberg 1, 14476, Golm, Germany

  3. C. Lacorte

    Present address: Laboratory of Virology, Department of Plant Sciences, Wageningen University, Binnenhaven 11, 6709 PD, Wageningen, The Netherlands

Authors and Affiliations

  1. Embrapa Recursos Geneticos e Biotecnologia, Laboratorio de Transferencia de Genes, Parque Estacao Biologica (PqEB), 70.849-970, Brasilia-DF, Brazil

    C. Studart-Guimaraes, C. Lacorte & A. C. M. Brasileiro

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  1. C. Studart-Guimaraes
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  2. C. Lacorte
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  3. A. C. M. Brasileiro
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Correspondence to A. C. M. Brasileiro.

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Studart-Guimaraes, C., Lacorte, C. & Brasileiro, A.C.M. Evaluation of heterologous promoters in transgenic Populus tremula × P. alba plants. Biol Plant 50, 15–20 (2006). https://doi.org/10.1007/s10535-005-0069-7

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  • DOI: https://doi.org/10.1007/s10535-005-0069-7

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