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Hydroxamate siderophores of Scedosporium apiospermum

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Abstract

Scedosporium apiospermum is an emerging pathogen colonizing the airways of patients with cystic fibrosis and causing severe infections in immunocompromised hosts. In order to improve our knowledge on the pathogenic mechanisms of this fungus, we investigated the production of siderophores. Cultivation on CAS medium and specific assays for different classes of siderophores suggested the secretion of hydroxamates. A maximal production was obtained by cultivation of the fungus at alkaline pH in an iron-restricted liquid culture medium. Siderophores were then extracted from the culture filtrate by liquid/liquid extraction, and separated by reverse phase high performance liquid chromatography. Two siderophores, dimerumic acid and Nα-methyl coprogen B, were identified by electrospray ionization-mass spectrometry and MS–MS fragmentation. Finally, comparison of various strains suggested a higher production of Nα-methyl coprogen B by clinical isolates of respiratory origin. Studies are initiated in order to determine the potential usefulness of these siderophores as diagnostic markers of scedosporiosis.

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Acknowledgments

We thank S. Fournier from the Plateforme d’Ingénierie et Analyses Moléculaires of Angers University for the LC–MS measurements. During this study, Samuel Bertrand was recipient of a grant from “Conseil Général du Maine et Loire, France”.

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Authors and Affiliations

  1. Laboratoire des Substances Naturelles et Analogues Structuraux, UPRES-EA 921, IFR 149 QUASAV, UFR de Pharmacie et Ingénierie de la Santé, Université d’Angers, 16 Bd Daviers, 49000, Angers, France

    Samuel Bertrand, Anne Landreau, Pascal Richomme & Olivier Duval

  2. Groupe d’Etude des Interactions Hôte-Pathogène, UPRES-EA 3142, IFR 132, Université d’Angers, 16 Bd Daviers, 49000, Angers, France

    Gérald Larcher & Jean-Philippe Bouchara

  3. Laboratoire de Parasitologie-Mycologie, Centre Hospitalier Universitaire, 4 rue Larrey, 49933, Angers cedex 9, France

    Gérald Larcher & Jean-Philippe Bouchara

Authors
  1. Samuel Bertrand
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  2. Gérald Larcher
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  3. Anne Landreau
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  4. Pascal Richomme
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  5. Olivier Duval
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  6. Jean-Philippe Bouchara
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Corresponding author

Correspondence to Gérald Larcher.

Additional information

SB, GL, AL and JPB are members of the ECMM (European Confederation of Medical Mycology)/ISHAM (International Society for Human and Animal Mycology) Working group on Pseudallescheria/Scedosporium infections, and of the ISHAM working group on fungal respiratory infections in cystic fibrosis.

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Bertrand, S., Larcher, G., Landreau, A. et al. Hydroxamate siderophores of Scedosporium apiospermum . Biometals 22, 1019–1029 (2009). https://doi.org/10.1007/s10534-009-9253-0

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  • DOI: https://doi.org/10.1007/s10534-009-9253-0

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