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The effects of lactoferrin in a rat model of catecholamine cardiotoxicity

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Abstract

Lactoferrin is recently under intense investigation because of its proposed several pharmacologically positive effects. Based on its iron-binding properties and its physiological presence in the human body, it may have a significant impact on pathological conditions associated with iron-catalysed reactive oxygen species (ROS). Its effect on a catecholamine model of myocardial injury, which shares several pathophysiological features with acute myocardial infarction (AMI) in humans, was examined. Male Wistar rats were randomly divided into four groups according to the received medication: control (saline), isoprenaline (ISO, 100 mg kg−1 s.c.), bovine lactoferrin (La, 50 mg kg−1 i.v.) or a combination of La + ISO in the above-mentioned doses. After 24 h, haemodynamic functional parameters were measured, a sample of blood was withdrawn and the heart was removed for analysis of various parameters. Lactoferrin premedication reduced some impairment caused by ISO (e.g. a stroke volume decrease, an increase in peripheral resistance and calcium overload). These positive effects were likely to have been mediated by the positive inotropic effect of lactoferrin and by inhibition of ROS formation due to chelation of free iron. The failure of lactoferrin to provide higher protection seems to be associated with the complexity of catecholamine cardiotoxicity and with its hydrophilic character.

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Acknowledgments

The authors wish to thank to Mrs. Anezka Kunova for her excellent technical support. This work was supported by grants of Charles University, No. 94/2006/C/FaF and 39207 C and by Research Project, No. MZO 001179906.

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Authors and Affiliations

  1. Faculty of Pharmacy in Hradec Králové, Department of Pharmacology and Toxicology, Charles University in Prague, Heyrovského 1203, 500 05, Hradec Kralove, Czech Republic

    Přemysl Mladěnka, Zuzana Bobrovová & Radomír Hrdina

  2. Faculty of Pharmacy in Hradec Králové, Department of Biological and Medical Sciences, Charles University in Prague, Hradec Kralove, Czech Republic

    Vladimír Semecký & Petr Nachtigal

  3. Faculty of Medicine in Hradec Králové, Institute of Clinical Biochemistry and Diagnostics, Charles University in Prague, Hradec Kralove, Czech Republic

    Jaroslava Vávrová, Magdaléna Holečková & Vladimir Palicka

  4. Faculty of Medicine in Hradec Králové, Department of Histology and Embryology, Charles University in Prague, Hradec Kralove, Czech Republic

    Yvona Mazurová

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  1. Přemysl Mladěnka
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  2. Vladimír Semecký
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Correspondence to Přemysl Mladěnka.

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Mladěnka, P., Semecký, V., Bobrovová, Z. et al. The effects of lactoferrin in a rat model of catecholamine cardiotoxicity. Biometals 22, 353–361 (2009). https://doi.org/10.1007/s10534-008-9172-5

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  • DOI: https://doi.org/10.1007/s10534-008-9172-5

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