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Responses of Rhodotorula sp. Y11 to cadmium

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Abstract

Some aspects of the cellular responses to cadmium were extensively investigated in the yeast Rhodotorula sp. Y11. Scanning electron microscopy indicated that accumulation of cadmium in the Y11 did not cause any visible effects on cell morphology. More than 20% yeast cells still showed viability after 15 h of cadmium accumulation under 100 mg l−1 cadmium concentration, and transmission electron microscopy analysis showed that plasmolysis and thickened cell wall were not observed in all of the cells. In the presence of cadmium, the activities of superoxide dismutase (SOD) and catalase (CAT) were all greater than the control, but the increase was in a dose-independent manner. Changes in SOD and CAT activities were also dependent on the time of exposure. Therefore, it suggests that antioxidative defenses play an important role in cadmium tolerance in Rhodotorula sp. Y11. Nondenaturing polyacrylamide gels revealed only one SOD isoforms in Y11 even under exposure to cadmium.

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Acknowledgements

This study was financially supported by the Major State Basic Research Development Program: Study on the Environmental Pollution Mechanisms and Control Principles in Beijing and Surrounding Area (G1999045709) and Tai Hu Project (No. BK 2007741) from Jiangsu Science and Technology.

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  1. College of Biological Sciences, Key Laboratory of Agro-Microbial Resource and Application, Ministry of Agriculture, China Agricultural University, Beijing, 100094, People’s Republic of China

    ZhiJian Li & HongLi Yuan

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  1. ZhiJian Li
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Correspondence to HongLi Yuan.

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Li, Z., Yuan, H. Responses of Rhodotorula sp. Y11 to cadmium. Biometals 21, 613–621 (2008). https://doi.org/10.1007/s10534-008-9147-6

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