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Biodegradation of 4-bromophenol by Arthrobacter chlorophenolicus A6 in batch shake flasks and in a continuously operated packed bed reactor

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Abstract

The present study investigated growth and biodegradation of 4-bromophenol (4-BP) by Arthrobacter chlorophenolicus A6 in batch shake flasks as well as in a continuously operated packed bed reactor (PBR). Batch growth kinetics of A. chlorophenolicus A6 in presence of 4-BP followed substrate inhibition kinetics with the estimated biokinetic parameters value of μ max = 0.246 h−1, K i = 111 mg L−1, K s  = 30.77 mg L−1 and K = 100 mg L−1. In addition, variations in the observed and theoretical biomass yield coefficient and maintenance energy of the culture were investigated at different initial 4-BP concentration. Results indicates that the toxicity tolerance and the biomass yield of A. chlorophenolicus A6 towards 4-BP was found to be poor as the organism utilized the substrate mainly for its metabolic maintenance energy. Further, 4-BP biodegradation performance by the microorganism was evaluated in a continuously operated PBR by varying the influent concentration and hydraulic retention time in the ranges 400–1,200 mg L−1 and 24–7.5 h, respectively. Complete removal of 4-BP was achieved in the PBR up to a loading rate of 2,276 mg L−1 day−1.

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Acknowledgments

The authors acknowledge the support received from the Centre for the Environment, Indian Institute of Technology Guwahati, for carrying out this research work.

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Authors and Affiliations

  1. Environmental Science and Technology Section (ITER), Department of Chemistry, S‘O’A University, Bhubaneswar, 751030, Orissa, India

    Naresh Kumar Sahoo

  2. Department of Biotechnology, Indian Institute of Technology Guwahati, Guwahati, 781039, India

    Kannan Pakshirajan

  3. Department of Civil Engineering, Indian Institute of Technology Guwahati, Guwahati, 781039, India

    Pranab Kumar Ghosh

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  1. Naresh Kumar Sahoo
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Correspondence to Naresh Kumar Sahoo.

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Sahoo, N.K., Pakshirajan, K. & Ghosh, P.K. Biodegradation of 4-bromophenol by Arthrobacter chlorophenolicus A6 in batch shake flasks and in a continuously operated packed bed reactor. Biodegradation 25, 265–276 (2014). https://doi.org/10.1007/s10532-013-9658-x

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