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Isolation and characterization of bacteria from mercury contaminated sites in Rio Grande do Sul, Brazil, and assessment of methylmercury removal capability of a Pseudomonas putida V1 strain

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Abstract

Methylmercury (MeHg) is one of the most dangerous heavy metal for living organisms that may be found in environment. Given the crescent industrialization of Brazil and considering that mercury is a residue of several industrial processes, there is an increasing need to encounter and develop remediation approaches of mercury contaminated sites. The aim of this study was to isolate and characterize methylmercury resistant bacteria from soils and sludge sewage from Rio Grande do Sul, Brazil. Sixteen bacteria were isolated from these contaminated sites and some isolates were highly resistant to methylmercury (>8.7 μM). All the isolates were identified by 16S rDNA. Pseudomonas putida V1 was able to volatilize approximately 90 % of methylmercury added to growth media and to resist to copper, lead, nickel, chromate, zinc, cobalt, manganese and barium. In the presence of high concentrations of methylmercury (12 μM), cell growth was limited, but P. putida V1 was still able to remove up to 29 % of this compound from culture medium. This bacterium removed an average of 77 % of methylmercury from culture medium with pH in the range 4.0–6.0. In addition, methylmercury was efficiently removed (>80 %) in temperature of 21–25 °C. Polymerase chain reactions indicated the presence of merA but not merB in P. putida V1. The growth and ability of P. putida V1 to remove methylmercury in a wide range of pH (4.0 and 8.0) and temperature (10–35 °C), its tolerance to other heavy metals and ability to grow in the presence of up to 11.5 μM of methylmercury, suggest this strain as a new potential resource for degrading methylmercury contaminated sites.

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Acknowledgments

This Project was supported by CAPES (Coordenação de Aperfeiçoamento de Pessoal de Nível Superior) and CNPq (Conselho Nacional de Desenvolvimento Científico e Tecnológico), Brazil. The authors are grateful to T. Barkay (Rutgers University) for critical reading this manuscript and for allowing us to perform PCR reaction (mer genes).

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Authors and Affiliations

  1. Department of Soil Science, Federal University of Rio Grande do Sul, UFRGS, Avenida Bento Gonçalves, 7712, Porto Alegre, RS, 91540-000, Brazil

    Lucélia Cabral, Clésio Gianello & Flávio Anastácio Oliveira Camargo

  2. Department of Microbiology, Instituto de Ciências Básicas da Saúde, Federal University of Rio Grande do Sul, Rua Sarmento Leite, 500, Porto Alegre, RS, 90050-170, Brazil

    Patrícia Giovanella & Fátima Menezes Bento

  3. Centro de Engenharias (CENG), Federal University of Pelotas, Av. Almirante Barroso, 1734. Centro., Pelotas, RS, 96010-280, Brazil

    Robson Andreazza

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  1. Lucélia Cabral
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Correspondence to Lucélia Cabral or Flávio Anastácio Oliveira Camargo.

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Cabral, L., Giovanella, P., Gianello, C. et al. Isolation and characterization of bacteria from mercury contaminated sites in Rio Grande do Sul, Brazil, and assessment of methylmercury removal capability of a Pseudomonas putida V1 strain. Biodegradation 24, 319–331 (2013). https://doi.org/10.1007/s10532-012-9588-z

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