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Isolation of a malachite green-degrading Pseudomonas sp. MDB-1 strain and cloning of the tmr2 gene

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Abstract

The release of malachite green, a commonly used triphenylmethane dye, into the environment is causing increasing concern due to its toxicity, mutagenicity, and carcinogenicity. A bacterial strain that could degrade malachite green was isolated from the water of an aquatic hatchery. It was identified as a Pseudomonas sp. based on the morphological, physiological, and biochemical characteristics, as well as the analysis of 16S rRNA gene sequence and designated as MDB-1. This strain was capable of degrading both malachite green and leucomalachite green, as well as other triphenylmethane dyes including Crystal Violet and Basic Fuchsin. The gene tmr2, encoding the triphenylmethane reductase from MDB-1, was cloned, sequenced and effectively expressed in E. coli. These results highlight the potential of this bacterium for the bioremediation of aquatic environments contaminated by malachite green.

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Acknowledgments

This work was supported by Agricultural Technology Transfer Programs (grant 2008) and National Program for Basic S and T Platform Construction (2005DKA21201-2).

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Authors and Affiliations

  1. Key Laboratory of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, College of Life Sciences, Nanjing Agricultural University, 210095, Nanjing, People’s Republic of China

    Lian-tai Li, Qing Hong, Xin Yan, Gui-hua Fang, Shinawar Waseem Ali & Shun-peng Li

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  1. Lian-tai Li
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  2. Qing Hong
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  3. Xin Yan
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  4. Gui-hua Fang
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  5. Shinawar Waseem Ali
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  6. Shun-peng Li
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Correspondence to Shun-peng Li.

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Lian-tai Li and Qing Hong contributed equally to this work.

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Li, Lt., Hong, Q., Yan, X. et al. Isolation of a malachite green-degrading Pseudomonas sp. MDB-1 strain and cloning of the tmr2 gene. Biodegradation 20, 769–776 (2009). https://doi.org/10.1007/s10532-009-9265-z

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  • DOI: https://doi.org/10.1007/s10532-009-9265-z

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