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Potential Applications in Sewage Bioremediation of the Highly Efficient Pyridine-Transforming Paenochrobactrum sp.

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Abstract

A pyridine-transforming strain P2 was isolated from sewage collected from Guangzhou oil stain field(China).According to the system analysis, it was identified as a member of the genus Paenochrobactrum. Comparative phylogenetic analysis based on the 16S rRNA and recA gene sequence showed that the isolate displayed the highest gene sequence similarity with members of the genus Paenochrobactrum, with Paenochrobactrum glaciei pi26T as the most closely related phylogenetic species (The similarity of gene sequence of gene sequence was 100 and 96.1% for 16S rRNA and recA, respectively).The G+C content of the genomic DNA of strain P2 was 57.2 ± 9.7%. The morphological and biochemical properties and DNA-DNA hybridization experiments revealed that strain P2 shows only 32.7 ± 2.6 to 42.7 ± 1.9% reassociation with the other Paenochrobactrum species. This strain could completely degrade 500 mg/L pyridine within 8 h at 30°C. The laboratory scale experiments of pyridine bioremediation of artificial mixed wastewater revealed that the removal of pyridine significantly increased after inoculation of bacteria. The strain P2 is a promising strain for the remediation of pyridine-contaminated wastewater.

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Funding

This work was supported by the National Science Foundation of China (NSFC 31500076), Science and Technology Program of Guangzhou, China (20190401016) and Guangzhou Municipal Science and Technology Project (20191A011063).

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    Authors and Affiliations

    1. Department of Environmental Health, Guangdong Provincial Key Laboratory of Tropical Disease Research, School of Public Health, Southern Medical University, 510515, Guangzhou, China

      X. Zhao, L. Chen, Q. Ren, Z. Wu, S. Fang, Y. Jiang, J. Wu & G. Zhang

    2. Guangzhou Center for Disease Control and Prevention, 510440, Guangzhou, China

      Y. Chen, Y. Zhong & D. Wang

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    1. X. Zhao
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    2. L. Chen
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    X. Zhao and L. Chen contributed equally to this work.

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    Correspondence to G. Zhang.

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    Authors declare that they have no conflict of interest. This article does not contain any studies involving animals or human participants performed by any of the authors.

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    Zhao, X., Chen, L., Ren, Q. et al. Potential Applications in Sewage Bioremediation of the Highly Efficient Pyridine-Transforming Paenochrobactrum sp.. Appl Biochem Microbiol 57, 344–350 (2021). https://doi.org/10.1134/S0003683821030145

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