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Laboratory scale cultivation of Salinispora tropica in shake flasks and mechanically stirred bioreactors

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Abstract

Objective

Marine actinomycetes from the genus Salinispora have an unexploited biotechnological potential. To accurately estimate their application potential however, data on their cultivation, including biomass growth kinetics, are needed but only incomplete information is currently available.

Results

This work provides some insight into the effect of temperature, salinity, nitrogen source, glucose concentration and oxygen supply on growth rate, biomass productivity and yield of Salinispora tropica CBN-440T. The experiments were carried out in unbaffled shake flasks and agitated laboratory-scale bioreactors. The results show that the optimum growth temperature lies within the range 28–30 °C, salinity is close to sea water and the initial glucose concentration is around 10 g/L. Among tested nitrogen sources, yeast extract and soy peptone proved to be the most suitable. The change from unbaffled to baffled flasks increased the volumetric oxygen transfer coefficient (kLa) as did the use of agitated bioreactors. The highest specific growth rate (0.0986 h−1) and biomass productivity (1.11 g/L/day) were obtained at kLa = 28.3 h−1. A further increase in kLa was achieved by increasing stirrer speed, but this led to a deterioration in kinetic parameters.

Conclusions

Improvement of S. tropica biomass growth kinetics of was achieved mainly by identifying the most suitable nitrogen sources and optimizing kLa in baffled flasks and agitated bioreactors.

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Funding

This research was supported by the Ministry of Education, Youth and Sports of the Czech Republic, Ministry of Agriculture of the Czech Republic (institutional support MZE-RO1918), and by Insubria University “Fondo di Ateneo per la Ricerca” 2017–2018.

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Authors and Affiliations

  1. Department of Biotechnology, University of Chemistry and Technology Prague, Technicka 5, 166 28, Prague, Czech Republic

    Zuzana Jezkova, Tomas Potocar, Martin Halecky & Tomas Branyik

  2. Department of Biotechnology and Life Sciences, University of Insubria, Via JH Dunant 3, 21100, Varèse, Italy

    Elisa Binda & Flavia Marinelli

  3. Research Institute of Brewing and Malting, Lipova 15, 120 44, Prague, Czech Republic

    Tomas Branyik

Authors
  1. Zuzana Jezkova
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  2. Elisa Binda
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  3. Tomas Potocar
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  4. Flavia Marinelli
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  5. Martin Halecky
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  6. Tomas Branyik
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Correspondence to Tomas Branyik.

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Jezkova, Z., Binda, E., Potocar, T. et al. Laboratory scale cultivation of Salinispora tropica in shake flasks and mechanically stirred bioreactors. Biotechnol Lett 43, 1715–1722 (2021). https://doi.org/10.1007/s10529-021-03121-1

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  • DOI: https://doi.org/10.1007/s10529-021-03121-1

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