Abstract
Objective
Immune response initiation and regulation require activation of dendritic cells (DCs). However, the mechanism by which ferritin, a carrier for immunogen, induces DCs maturation remains unclear.
Results
Recombinant ferritin nanoparticle (RFNp), were prepared through the baculovirus expression vector system, formed spherical and hollow cage-liked proteins with a diameter of approximately 12.17 ± 0.87 nm. They induced bone marrow-derived DC (BMDC) maturation via surface molecules up-regulation of (MHC II, CD80, CD86 and CD40), increased pro-inflammatory cytokines production (IL-6, IL-12, TNF-α, and IFN-γ), and decreased antigen capturing capacity. They positively regulated IκBα and NF-κB (p65) phosphorylation, and facilitate NF-κB (p65) translocation into mature BMDCs nuclei. Following pre-treatment of RFNp-treated BMDCs with TLR4 and NF-κB (p65) inhibitors, respectively, surface molecule expression, pro-inflammatory cytokines production, and IκBα and NF-κB (p65) activities were suppressed. RFNp-treated BMDCs can also facilitate T-cell proliferation and differentiation into Th1 and Th2.
Conclusion
RFNps induced DCs maturation lends the potential application of RFNps as carrier platforms in DC-based vaccine.
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This work was supported by the Agriculture Research System of China (Grant Number CARS-36).
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Qu, Z., Guo, Y., Li, M. et al. Recombinant ferritin nanoparticles can induce dendritic cell maturation through TLR4/NF-κB pathway. Biotechnol Lett 42, 2489–2500 (2020). https://doi.org/10.1007/s10529-020-02944-8
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DOI: https://doi.org/10.1007/s10529-020-02944-8