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Screening and identification of a specific peptide binding to cervical cancer cells from a phage-displayed peptide library

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Abstract

Objectives

To screen and identify the probe markers specifically binding to human cervical cancer, a phage-displayed 12-mer peptide library was used for biopanning of SiHa cells.

Results

After four rounds of whole-cell subtraction biopanning, the phage recovery was 21-fold higher (from 3.9 × 10−5 to 8.3 × 10−4) than that of the first round, and specific phage clones were significantly enriched. 57 randomly selected phage clones were tested by ELISA, and 36 phage clones were identified as positive clones. After sequencing of positive clones, six different peptide sequences were obtained and CSP3 showed best affinity and specificity to SiHa cells via immunofluorescence assay.

Conclusions

Peptide, CSP3, bound to SiHa cells specifically and sensitively. It may be a potential candidate for molecular imaging detection and targeting therapy of cervical cancer.

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Acknowledgements

This work was supported by the National Natural Science Foundation of China (No. 81172359).

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Authors and Affiliations

  1. Department of Obstetrics and Gynecology, Xijing Hospital, The Fourth Military Medical University, Xi’an, 710032, Shaanxi, China

    Chunyan Li, Jianfang Zhang & Biliang Chen

  2. Medical College of Yan’an University, Yan’an, 716000, Shaanxi, China

    Ning Gao

  3. Co-Innovation Center for Qinba Region’s Sustainable Development, Shaanxi Normal University, 199 South Chang’an Road, Xi’an, 710062, Shaanxi, China

    Qinqin Xue, Ni Ma, Yuqin Hu & Yingchun Hou

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  1. Chunyan Li
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  2. Ning Gao
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  3. Qinqin Xue
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  4. Ni Ma
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  5. Yuqin Hu
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  6. Jianfang Zhang
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  7. Biliang Chen
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  8. Yingchun Hou
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Correspondence to Yingchun Hou.

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Li, C., Gao, N., Xue, Q. et al. Screening and identification of a specific peptide binding to cervical cancer cells from a phage-displayed peptide library. Biotechnol Lett 39, 1463–1469 (2017). https://doi.org/10.1007/s10529-017-2381-7

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  • DOI: https://doi.org/10.1007/s10529-017-2381-7

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