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Micropropagation of Ajuga species: a mini review

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Abstract

The genus Ajuga L., belonging to Lamiaceae family, is widespread. The demand for Ajuga species has risen sharply because of their medicinal, ornamental, and pharmacological properties. These wide-ranging plants are being rapidly depleted due to over-collection for ornamental and medicinal purposes, as well as by habitat destruction and deforestation. Ajuga boninsimae, A. bracteosa, A. ciliate, A. genevensis, A. incisa, A. makinoi, A. multiflora, A. pyramidalis, A. shikotanensis, A. reptans, and A. vestita are categorized and protected as endangered plants. In vitro plant culture has therefore emerged for the conservation and mass clonal propagation of rare plants. This mini-review covers the current in vitro scenario in the propagation of Ajuga species. Adventitious or axillary shoots are initiated on the leaf, petiole and internodes, as well as roots, nodes, and shoot tip explants. Shoot induction is predominantly dependent on plant growth regulators added to the culture medium. Full- or half-strength Murashige and Skoog medium with or without auxin is used for in vitro rooting. Rooted shoots need to be acclimatized in the greenhouse with an estimated 82–100% survival rate.

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Acknowledgements

This article was supported by the KU Research Professor Program of Konkuk University.

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Authors and Affiliations

  1. Department of Bioresource Engineering, Sejong University, Seoul, South Korea

    Han Yong Park

  2. Department of Bioresources and Food Science, Konkuk University, Seoul, South Korea

    Doo Hwan Kim & Iyyakkannu Sivanesan

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  1. Han Yong Park
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  2. Doo Hwan Kim
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Correspondence to Iyyakkannu Sivanesan.

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Park, H.Y., Kim, D.H. & Sivanesan, I. Micropropagation of Ajuga species: a mini review. Biotechnol Lett 39, 1291–1298 (2017). https://doi.org/10.1007/s10529-017-2376-4

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