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Desulfurization with Thialkalivibrio versutus immobilized on magnetic nanoparticles modified with 3-aminopropyltriethoxysilane

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Abstract

Objective

Thialkalivibrio versutus D301 cells were immobilized on Fe3O4 nanoparticles (NPs) synthesized by an improved chemical coprecipitation method and modified with 3-aminopropyltriethoxysilane (APTES), then the immobilized cells were used in sulfur oxidation.

Results

The prepared Fe3O4–APTES NPs had a narrow size distribution (10 ± 2 nm) and were superparamagnetic, with a saturation magnetization of 60.69 emu/g. Immobilized cells had a saturation magnetization of 34.95 emu/g and retained superparamagnetism. The optimum conditions for cell immobilization were obtained at pH 9.5 and 1 M Na+. The immobilization capacity of Fe3O4–APTES NPs was 7.15 g DCW/g-NPs that was 2.3-fold higher than that of Fe3O4 NPs. The desulfurization efficiency of the immobilized cells was close to 100%, having the same sulfur oxidation capacity as free cells. Further, the immobilized cells could be reused at least eight times, retaining more than 85% of their desulfurization efficiency.

Conclusion

Immobilization of cells with the modified magnetic NPs efficiently increased cell controllability, have no effect on their desulfurization activity and could be effectively used in large-scale industrial applications.

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Supporting information

Supplementary Fig. 1—ATR-FTIR spectra of Fe3O4 NPs and Fe3O4–APTES NPs.

Supplementary Fig. 2—TGA of Fe3O4 NPs and Fe3O4–APTES NPs.

Supplementary Fig. 3—Magnetization curves of Fe3O4 NPs, Fe3O4–APTES NPs and cells immobilized on Fe3O4–APTES NPs.

Supplementary Fig. 4—Effect of Na+ concentration for cells immobilization.

Supplementary Fig. 5—Effect of solution pH for cells immobilization.

Supplementary Fig. 6—Effect of nanoparticles dosage for cells immobilization.

Supplementary Fig. 7—Adsorption isotherms of Thialkalivibrio versutus D301 cells on Fe3O4–APTES NPs fitted with Langmuir and Freundlich model.

Supplementary Fig. 8—Zeta potentials of Fe3O4 NPs, Fe3O4–APTES NPs and Thialkalivibrio versutus D301 cells.

Author information

Author notes

    Authors and Affiliations

    1. Key Laboratory of Green Process and Engineering, State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing, 100190, China

      Tingzhen Mu, Jiangnan Tian, Maohua Yang, Chen Guo & Jianmin Xing

    2. School of Materials Science and Engineering, University of Science and Technology Beijing, Beijing, 100083, China

      Jixiang Zhao & Yueping Guan

    3. University of Chinese Academy of Sciences, Beijing, 100049, China

      Jiangnan Tian, Chen Guo & Jianmin Xing

    Authors
    1. Tingzhen Mu
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    2. Jixiang Zhao
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    3. Yueping Guan
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    4. Jiangnan Tian
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    5. Maohua Yang
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    6. Chen Guo
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    7. Jianmin Xing
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    Corresponding author

    Correspondence to Jianmin Xing.

    Additional information

    Tingzhen Mu and Jixiang Zhao have contributed equally to this work.

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    Supplementary material 1 (DOCX 593 kb)

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    Mu, T., Zhao, J., Guan, Y. et al. Desulfurization with Thialkalivibrio versutus immobilized on magnetic nanoparticles modified with 3-aminopropyltriethoxysilane. Biotechnol Lett 39, 865–871 (2017). https://doi.org/10.1007/s10529-017-2317-2

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    • DOI: https://doi.org/10.1007/s10529-017-2317-2

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