Abstract
Objective
Methionine is a valid target for the treatment of cancer and to achieve in vivo imaging and early diagnosis of tumors, we have synthesized near-infrared (NIR) fluorochrome IR-822-labeled methionine (IR-822-Met).
Results
NIR fluorescent dye IR-822 was conjugated with methionine through its amide bond. It had low toxicity to normal cell/tissues. In vitro and in vivo studies demonstrated its high targeting capability to tumors. The results support the potential of using ligand-modified methionine probe for tumor diagnosis and targeted therapy. The probe also exhibited good photostability, and excellent cell membrane permeability.
Conclusion
IR-822-Met is a promising imaging agent for tumor diagnosis, especially in their early stage.
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Acknowledgements
This work was supported by Shandong Provincial Natural Science Foundation (ZR2016BL16, ZR2016CL01), China, National High-tech Research and Development Project (863 Project, 2013AA032205), Industry Project of Jiangsu Science-Technology Support Plan (BE2013840) and Science and Technology Development Program of Suzhou (ZXY201412).
Supporting information
Supplementary Figure 1—1H-NMR and MS of IR-822.
Supplementary Figure 2—1H-NMR and MS of IR-822-Met.
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Sun, C., Zhang, H., Du, W. et al. Synthesis of a Novel IR-822-Met near-infrared probe for in vivo tumor diagnosis. Biotechnol Lett 39, 491–499 (2017). https://doi.org/10.1007/s10529-016-2275-0
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DOI: https://doi.org/10.1007/s10529-016-2275-0