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A novel chimeric influenza virosome containing Vesicular stomatitis G protein as a more efficient gene delivery system

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Abstract

Objectives

To enhance the efficiency of influenza virosome-mediated gene delivery by engineering this virosome.

Results

A novel chimeric influenza virosome was constructed containing the glycoprotein of Vesicular stomatitis virus (VSV-G), along with its own hemagglutinin protein. To optimize the transfection efficiency of both chimeric and influenza cationic virosomes, HEK cells were transfected with plasmid DNA and virosomes and the transfection efficiency was assessed by FACS analysis. The chimeric virosome was significantly more efficient in mediating transfection for all amounts of DNA and virosomes compared to the influenza virosome.

Conclusions

Chimeric influenza virosome, including VSV-G, is superior to the conventional influenza virosome for gene delivery.

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Acknowledgment

This work was supported by a grant from Pasteur Institute, Tehran, Iran.

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Authors and Affiliations

  1. Influenza Research Lab, Department of Virology, Pasteur Institute of Iran, No. 358, 12th Farwardin Ave, Jomhhoori St, Tehran, Iran

    Yahya Mohammadzadeh, Narges Rasouli, Mohammad Hasan Samiee Aref, Nasim Sadat Seyed Tabib, Peyvand Biglari, Maryam Saleh, Mansoureh Tabatabaeian, Masoumeh Tavassoti Kheiri & Abbas Jamali

  2. Department of Hepatitis and AIDS, Pasteur Institute of Iran, Tehran, Iran

    Asghar Abdoli

Authors
  1. Yahya Mohammadzadeh
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  2. Narges Rasouli
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  3. Mohammad Hasan Samiee Aref
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  4. Nasim Sadat Seyed Tabib
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  5. Asghar Abdoli
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  6. Peyvand Biglari
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  7. Maryam Saleh
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  8. Mansoureh Tabatabaeian
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  9. Masoumeh Tavassoti Kheiri
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  10. Abbas Jamali
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Corresponding author

Correspondence to Abbas Jamali.

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Conflict of Interest

The authors declare that there are no conflicts of interest

Additional information

Yahya Mohammadzadeh and Narges Rasouli are equal first authors.

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Mohammadzadeh, Y., Rasouli, N., Aref, M.H.S. et al. A novel chimeric influenza virosome containing Vesicular stomatitis G protein as a more efficient gene delivery system. Biotechnol Lett 38, 1321–1329 (2016). https://doi.org/10.1007/s10529-016-2108-1

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  • DOI: https://doi.org/10.1007/s10529-016-2108-1

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