Abstract
Objectives
A chaperonin, PsyGroELS, from the Antarctic psychrophilic bacterium Psychrobacter sp. PAMC21119, was examined for its role in cold adaptation when expressed in a mesophilic Escherichia coli strain.
Results
Growth of E. coli harboring PsyGroELS at 10 °C was increased compared to the control strain. A co-expression system using PsyGroELS was developed to increase productivity of the psychrophilic enzyme PsyEst9. PsyEst9 was cloned and expressed using three E. coli variants that co-expressed GroELS from PAMC21119, E. coli, or Oleispira antarctica RB8T. Co-expression with PsyGroELS was more effective for the production of PsyEst9 compared tothe other chaperonins.
Conclusion
PsyGroELS confers cold tolerance to E. coli, and shows potential as an effective co-expression system for the stable production of psychrophilic proteins.
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Acknowledgments
This work was supported by Development of Cold-adapted Chaperonin GroEL/ES (PE13110), and the Antarctic Organisms: Cold-Adaptation Mechanisms (PE15070) funded from the Korea Polar Research Institute.
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Kim, HW., Wi, A.R., Jeon, B.W. et al. Cold adaptation of a psychrophilic chaperonin from Psychrobacter sp. and its application for heterologous protein expression. Biotechnol Lett 37, 1887–1893 (2015). https://doi.org/10.1007/s10529-015-1860-y
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DOI: https://doi.org/10.1007/s10529-015-1860-y