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Spray-painted human fibronectin coating as an effective strategy to enhance graft ligamentization of a polyethylene terephthalate artificial ligament

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Abstract

To enhance graft ligamentization after anterior cruciate ligament (ACL) reconstruction, human fibronectin (FN) was coated on polyethylene terephthalate (PET) ligaments by spray painting. The FN-coated PET ligaments were investigated in vitro using rat mesenchymal stromal cells (MSCs). MSCs cultured on FN-coated grafts resulted in similar cell densities and amounts of proliferating cells with control grafts without coating. The FN-coated group not only gave rise to MSC-derived collagen-like tissues but also enhanced the expression of collagen-I gene. Furthermore, rat ACL reconstruction models were used to evaluate the effect of the FN coating in vivo. The FN coating significantly promoted new ligament tissue regeneration into the graft fibers. In conclusion, sprayed FN coating had a positive effect to enhance graft ligamentization of PET artificial ligament.

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Acknowledgments

This project was subsidized by the 973 Project (No. 2009CB930000) from the Ministry of Science and Technology of China. The Nano project of Shanghai Municipal Science and Technology Commission (1052nm03701, 11nm0504900). Young Project of National Natural Science Foundation of China (81000816). The Project of Shanghai Municipal Science and Technology Commission (11JC1401700).

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The authors report no conflict of interest.

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Authors and Affiliations

  1. Department of Sports Medicine, Huashan Hospital, No. 12, Wulumuqi Zhong Road, Shanghai, 200040, People’s Republic of China

    Hong Li, Yunsheng Ge & Shiyi Chen

  2. Department of Orthopaedics, Zhongshan Hospital, Fudan University, Shanghai, People’s Republic of China

    Chen Chen

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  1. Hong Li
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  2. Chen Chen
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  3. Yunsheng Ge
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Correspondence to Shiyi Chen.

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Li, H., Chen, C., Ge, Y. et al. Spray-painted human fibronectin coating as an effective strategy to enhance graft ligamentization of a polyethylene terephthalate artificial ligament. Biotechnol Lett 36, 1079–1088 (2014). https://doi.org/10.1007/s10529-013-1447-4

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  • DOI: https://doi.org/10.1007/s10529-013-1447-4

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