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Impact of heparin-binding domain of recombinant human osteocalcin-fibronectinIII9-14 on the osteoblastic cell response

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Abstract

Fibronectin (FN) containing a heparin-binding domain (HBD) and an Arg-Gly-Asp (RGD) domain can promote cell adhesion and proliferation compared to FN that contained only RGD. Here, we have engineered recombinant human osteocalcin (rhOC) with FN type III9-14 (rhOC-FNIII9-14) containing RGD and HBD to promote the cellular activity of MC3T3-E1 cells, including adhesion, proliferation, and differentiation. RhOC-FNIII9-14 significantly increased cell adhesion and proliferation of MC3T3-E1 cells compared to rhOC-FNIII9-10 (P < 0.05). Moreover, rhOC-FNIII9-14 showed osteogenic differentiation of MC3T3-E1 cells in mineralization activity and osteogenic gene expression.

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Acknowledgments

This work was supported by the Priority Research Centers Program (Grant#: 2009-0093829), the World Class University (WCU) program (Grant#: R31-10069), and the Basic Science Research program through the National Research Foundation (NRF) funded by the Ministry of Education, Science, and Technology (2010-0022628, 2013R1A1A2011375) and a grant from the Korea Healthcare Technology R&D Project, Ministry of Health & Welfare, Republic of Korea (HI11C0388), and an Inha University Grant.

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Authors and Affiliations

  1. Institute of Tissue Regeneration Engineering (ITREN), Dankook University, Cheonan, 330-714, South Korea

    Ye-Rang Yun & Hae-Won Kim

  2. Department of Nanobiomedical Science and WCU Research Center, Dankook University Graduate School, Cheonan, 330-714, South Korea

    Ye-Rang Yun & Hae-Won Kim

  3. Department of Biomaterials Science, School of Dentistry, Dankook University, Cheonan, 330-714, South Korea

    Hae-Won Kim

  4. Department of Biochemistry, Inha University School of Medicine, Inchon, 400-712, South Korea

    Jun-Hyeog Jang

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  1. Ye-Rang Yun
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  2. Hae-Won Kim
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  3. Jun-Hyeog Jang
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Correspondence to Jun-Hyeog Jang.

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Yun, YR., Kim, HW. & Jang, JH. Impact of heparin-binding domain of recombinant human osteocalcin-fibronectinIII9-14 on the osteoblastic cell response. Biotechnol Lett 35, 2213–2220 (2013). https://doi.org/10.1007/s10529-013-1334-z

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  • DOI: https://doi.org/10.1007/s10529-013-1334-z

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