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Construction and expression of a recombinant fibronectinIII10 protein for integrin-mediated cell adhesion

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Abstract

Specific sequences of cell-adhesive peptide/proteins are often required for the bio-inert synthetic polymers to improve cell adhesion. We have developed a recombinant fibronectin fragment (FNIII10), the central cell-binding domain containing RGD motif, to endow biomaterial surfaces with abilities to promote an integrin-mediated cell adhesion. Immobilized FNIII10 stimulated adhesion of MC3TC-E1 cells in an integrin-dependent manner.

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References

  • Akiyama SK, Yamada SS, Chen WT et al (1989) Analysis of fibronectin receptor function with monoclonal antibodies: roles in cell adhesion, migration, matrix assembly, and cytoskeletal organization. J Cell Biol 109:863–875

    Article  CAS  PubMed  Google Scholar 

  • Choung PH, Seo BM, Chung CP et al (2002) Synergistic activity of fibronectin and fibroblast growth factor receptors on neuronal adhesion and neurite extension through extracellular signal-regulated kinase pathway. Biochem Biophys Res Commun 295:898–902

    Article  CAS  PubMed  Google Scholar 

  • Clark EA, Brugge JS (1995) Integrins and signal transduction pathways: the road taken. Science 268:233–239

    Article  CAS  PubMed  Google Scholar 

  • Hynes RO (1992) Integrins: versatility, modulation, and signaling in cell adhesion. Cell 69:11–25

    Article  CAS  PubMed  Google Scholar 

  • Hynes RO (2002) Integrins: bidirectional, allosteric signaling machines. Cell 110:673–687

    Article  CAS  PubMed  Google Scholar 

  • Kang W, Park S, Jang JH (2008) Kinetic and functional analysis of the heparin-binding domain of fibronectin. Biotechnol Lett 30:55–59

    Article  CAS  PubMed  Google Scholar 

  • Lutolf MP, Hubbell JA (2005) Synthetic biomaterials as instructive extracellular microenvironments for morphogenesis in tissue engineering. Nat Biotechnol 23:47–55

    Article  CAS  PubMed  Google Scholar 

  • Ruoslahti E (1988) Fibronectin and its receptors. Annu Rev Biochem 57:375–413

    Article  CAS  PubMed  Google Scholar 

  • Schuler M, Owen GR, Hamilton DW et al (2006) Biomimetic modification of titanium dental implant model surfaces using the RGDSP-peptide sequence: a cell morphology study. Biomaterials 27:4003–4015

    Article  CAS  PubMed  Google Scholar 

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Acknowledgements

This study was supported by a grant of the Korea Healthcare technology R&D Project, Ministry for Health, Welfare and Family Affairs, Republic of Korea (A084458).

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Authors and Affiliations

  1. Department of Biomaterials Science, School of Dentistry and Institute of Tissue Regeneration Engineering (ITREN), Dankook University, Seoul, Korea

    Hae-Won Kim

  2. Biomaterials and Tissue Engineering Lab, Department of Nanobiomedical Science and WCU Research Center, Dankook University, Seoul, Korea

    Hae-Won Kim

  3. Department of Biochemistry, Inha University School of Medicine, Inchon, Korea

    Wonmo Kang, Eunyi Jeon & Jun-Hyeog Jang

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  1. Hae-Won Kim
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  2. Wonmo Kang
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  3. Eunyi Jeon
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  4. Jun-Hyeog Jang
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Correspondence to Jun-Hyeog Jang.

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Kim, HW., Kang, W., Jeon, E. et al. Construction and expression of a recombinant fibronectinIII10 protein for integrin-mediated cell adhesion. Biotechnol Lett 32, 29–33 (2010). https://doi.org/10.1007/s10529-009-0111-5

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  • DOI: https://doi.org/10.1007/s10529-009-0111-5

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