Abstract
We have investigated whether simultaneous modification of cofactor metabolism and glycerol in a strain of Saccharomyces cerevisiae can eliminate glycerol synthesis during ethanol production. Two strains, S812 (gpd1Δ gpd2Δ PGK1p-GLT1) and LE17 (gpd1Δ gpd2Δ PGK1p-GLT1 PGKp-STL1) were generated that showed a 8 and 8.2 % increase in the ethanol yield, respectively, compared to the wild type KAM-2 strain. The ethanol titer was improved from 90.4 g/l for KAM-2 to 97.6 g/l for S812 and 97.8 g/l for LE17, respectively. These results provide a new insight into rationalization of metabolic engineering strategies for improvement of ethanol yield through elimination of glycerol production.
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Acknowledgments
We would like to express our deepest appreciation to our Ph.D. promoter, Dr. Pingsheng MA, for his valuable and constructive advice on this study, which formed the basis of this paper. We are very grateful to Dr. T.L. Turner (University of Illinois at Urbana-Champaign) for his suggestions on the writings of our revised manuscript. This work was supported by the National Natural Science Foundation of China (Grant No. 30771108/C0702) and the Ministry of Science and Technology of China (“863”Program: 2012AA02A701).
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Jingyu Wang and Wen Liu contributed equally to this study.
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Wang, J., Liu, W., Ding, W. et al. Increasing ethanol titer and yield in a gpd1Δ gpd2Δ strain by simultaneous overexpression of GLT1 and STL1 in Saccharomyces cerevisiae . Biotechnol Lett 35, 1859–1864 (2013). https://doi.org/10.1007/s10529-013-1271-x
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DOI: https://doi.org/10.1007/s10529-013-1271-x