Abstract
Ramoplanins are lipopeptides effective against a wide range of Gram-positive pathogens. Ramoplanin A2 is in Phase III clinical trials. The structure–activity relationship of the unique 2Z,4E-fatty acid side-chain of ramoplanins indicates a significant contribution to the antimicrobial activities but ramoplanin derivatives with longer 2Z,4E-fatty acid side-chains are not easy to obtain by semi-synthetic approaches. To construct a strain that produces such analogues, an acyl-CoA ligase gene in a ramoplanin-producing Actinoplanes was inactivated and a heterologous gene from an enduracidin producer (Streptomyces fungicidicus) was introduced into the mutant. The resulting strain produced three ramoplanin analogues with longer alkyl chains, in which X1 was purified. The MIC value of X1 was ~0.12 μg/ml against Entrococcus sp. and was also active against vancomycin-resistant Staphylococcus aureus (MIC = 2 μg/ml).
Similar content being viewed by others
References
Bierman M, Logan R, O’Brien K, Seno ET, Rao RN, Schoner BE (1992) Plasmid cloning vectors for the conjugal transfer of DNA from Escherichia coli to Streptomyces spp. Gene 116:43–49
Cavalleri B, Pagani H, Volpe G, Selva E, Parenti F (1984) A-16686, a new antibiotic from Actinoplanes. I. Fermentation, isolation and preliminary physico-chemical characteristics. J Antibiot 37:309–317
Ciabatti R, Kettenring JK, Winters G, Tuan G, Zerilli L, Cavalleri B (1989) Ramoplanin (A-16686), a new glycolipodepsipeptide antibiotic III. Structure elucidation. J Antibiot 42:254–267
Ciabatti R, Maffioli SI, Panzone G, Canavesi A, Michelucci E, Tiseni PS, Marzorati E, Checchia A, Giannone M, Jabes D, Romano G, Brunati C, Candiani G, Castiglione F (2007) Synthesis and preliminary biological characterization of new semisynthetic derivatives of ramoplanin. J Med Chem 50:3077–3085
Finegold SM, John SS, Vu AW, Li CM, Molitoris D, Song Y, Liu C, Wexler HM (2004) In vitro activity of ramoplanin and comparator drugs against anaerobic intestinal bacteria from the perspective of potential utility in pathology involving bowel flora. Anaerobe 10:205–211
Gandolfi R, Marinelli F, Ragg E, Romano D, Molinari F (2012) Chemoenzymatic deacylation of ramoplanin. Bioorg Med Chem Lett 22:5283–5287
Hu Y, Helm JS, Chen L, Ye XY, Walker S (2003) Ramoplanin inhibits bacterial transglycosylases by binding as a dimer to lipid II. J Am Chem Soc 125:8736–8737
Kieser T, Bibb MJ, Buttner MJ, Chater KF, Hopwood DA (2000) Practical Streptomyces genetics. John Innes Foundation, Norwich
MacNeil DJ, Gewain KM, Ruby CL, Dezeny G, Gibbons PH, MacNeil T (1992) Analysis of Streptomyces avermitilis genes required for avermectin biosynthesis utilizing a novel integration vector. Gene 111:61–68
McCafferty DG, Cudic P, Frankel BA, Barkallah S, Kruger RG, Li W (2002) Chemistry and biology of the ramoplanin family of peptide antibiotics. Biopolymers 66:261–284
Nam J, Shin D, Rew Y, Boger DL (2007) Alanine scan of [L-Dap2] ramoplanin A2 aglycon: assessment of the importance of each residue. J Am Chem Soc 129:8747–8755
Pan HX, Li JA, Shao L, Zhu CB, Chen JS, Tang GL, Chen DJ (2013) Genetic manipulation revealing an unusual N-terminal region in a stand-alone non-ribosomal peptide synthetase involved in the biosynthesis of ramoplanins. Biotechnol Lett 35:107–114
Peláez T, Alcalá L, Alonso R, Martín-López A, García-Arias V, Marín M, Bouza E (2005) In vitro activity of ramoplanin against Clostridium difficile, including strains with reduced susceptibility to vancomycin or with resistance to metronidazole. Antimicrob Agents Chemother 49:1157–1159
Rajgarhia VB, Strohl WR (1997) Minimal Streptomyces sp. strain C5 daunorubicin polyketide biosynthesis genes required for aklanonic acid biosynthesis. J Bacteriol 179:2690–2696
Rew Y, Shin D, Hwang I, Boger DL (2004) Total synthesis and examination of three key analogues of ramoplanin: a lipoglycodepsipeptide with potent antibiotic activity. J Am Chem Soc 126:1041–1043
Rolston KV, Dholakia N, Ho DH, LeBlanc B, Dvorak T, Streeter H (1996) In-vitro activity of ramoplanin (a novel lipoglycopeptide), vancomycin, and teicoplanin against gram-positive clinical isolates from cancer patients. J Antimicrob Chemother 38:265–269
Somner EA, Reynolds PE (1990) Inhibition of peptidoglycan biosynthesis by ramoplanin. Antimicrob Agents Chemother 34:413–419
Yin X, Zabriskie TM (2006) The enduracidin biosynthetic gene cluster from Streptomyces fungicidicus. Microbiology 152:2969–2983
Acknowledgments
This work was supported by grants from the National Natural Science Foundation of China (81072557, 81172962, and 81202442), the Science and Technology Commission of Shanghai Municipality (11QB1406300) and the Ministry of Science and Technology of China (Grant No. 2009ZX09301-007).
Author information
Authors and Affiliations
Corresponding authors
Electronic supplementary material
Below is the link to the electronic supplementary material.
Rights and permissions
About this article
Cite this article
Pan, HX., Chen, ZZ., Shao, L. et al. Production of ramoplanin analogues by genetic engineering of Actinoplanes sp.. Biotechnol Lett 35, 1685–1692 (2013). https://doi.org/10.1007/s10529-013-1261-z
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10529-013-1261-z