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Verrucarin A, a protein synthesis inhibitor, induces growth inhibition and apoptosis in breast cancer cell lines MDA-MB-231 and T47D

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Abstract

Verrucarin A (VA), a protein synthesis inhibitor, derived from the pathogen fungus Myrothecium verrucaria, inhibits growth of leukemia cell lines and activates caspases and apoptosis and inflammatory signaling in macrophages. We have investigated VA-induced growth inhibition in breast cancer cells MDA-MB-231 and T47D and, particularly, the mechanism of VA-induced apoptosis. VA treatment brought about apoptotic cell death in a dose- and time-dependent manner which was associated with chromatin condensation, cell shrinkage, nuclear fragmentation and intracellular ROS production. Mitochondrial membrane depolarization, activation of caspase-3, down-regulation of Bcl-2 expression and up-regulation of Bax and p53 expression were observed. VA thus affects the viability of both the breast cancer cells by triggering ROS-mediated intrinsic mechanism of apoptosis.

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Acknowledgments

This work was supported by University Grants Commission, India, under the Special Assistance Programme (UGC–SAP)-Research Fellowship for Meritorious Students in Science (RFMS).

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The authors declare that there is no conflict of interest.

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Authors and Affiliations

  1. Department of Animal Science, School of Life Sciences, Bharathidasan University, Tiruchirappalli, 620 024, Tamil Nadu, India

    Kandasamy Palanivel, Veerasamy Kanimozhi & Balamuthu Kadalmani

  2. Mahatma Gandhi-Doerenkamp Centre (MGDC), Bharathidasan University, Tiruchirappalli, 620 024, Tamil Nadu, India

    Mohammad Abdulkader Akbarsha

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  1. Kandasamy Palanivel
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  2. Veerasamy Kanimozhi
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  3. Balamuthu Kadalmani
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  4. Mohammad Abdulkader Akbarsha
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Correspondence to Balamuthu Kadalmani.

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Palanivel, K., Kanimozhi, V., Kadalmani, B. et al. Verrucarin A, a protein synthesis inhibitor, induces growth inhibition and apoptosis in breast cancer cell lines MDA-MB-231 and T47D. Biotechnol Lett 35, 1395–1403 (2013). https://doi.org/10.1007/s10529-013-1238-y

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  • DOI: https://doi.org/10.1007/s10529-013-1238-y

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