Abstract
Purpose of work
Plants synthesize and accumulate secondary metabolites as defensive volatiles against diverse stresses. We aim to unravel the jasmonate-inducible volatile de novo synthetic metabolites in plants using a deuterium-labeling technique.
Jasmonic acid and its methyl ester (MeJA) are well-documented for inducing defensive volatiles. Here, we have developed an efficient deuterium oxide (D2O)-based labeling approach to determine the extent of de novo synthetic metabolites in a model plant A. bidentata bidentata. The labeling approach was demonstrated on quantitative profiling of terpene volatile organic compounds (VOCs) elicited by airborne MeJA in Achyranthes plants. We show, for the first time that airborne MeJA-elicited terpene VOCs are predominantly and differentially de novo synthesized except for a homoterpene, (3E)-4,8-dimethyl-1,3,7-nonatriene, which is weakly and least labelled with deuterium. D2O is therefore an efficient labeling source for investigating de novo synthetic metabolites of terpene VOCs in planta.
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Acknowledgments
ST appreciates Japan Society for the Promotion of Science for a research fund (JSPS; ID Number C-24580165). GKA appreciates Japan Society for the Promotion of Science (JSPS; ID Number S-10182) for research at NIAS and collaborations therein with ST and RR. RR acknowledges the great support of Professor Yoshihiro Shiraiwa (Provost, Faculty of Life and Environmental Sciences, University of Tsukuba) and Prof. Seiji Shioda and Dr. Tetsuo Ogawa (Department of Anatomy I, Showa University School of Medicine) in promoting interdisciplinary research and unselfish encouragement.
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Tamogami, S., Noge, K., Abe, M. et al. Deuterium labeling for investigating de novo synthesis of terpene volatiles in Achyranthes bidentata . Biotechnol Lett 35, 1247–1252 (2013). https://doi.org/10.1007/s10529-013-1201-y
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DOI: https://doi.org/10.1007/s10529-013-1201-y