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Enhanced biotransformation of 1,3-dichloro-2-propanol to epichlorohydrin via resin-based in situ product removal process

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Abstract

Biotransformation of 1,3-dichloro-2-propanol (DCP) to epichlorohydrin (ECH) by the whole cells of recombinant Escherichia coli expressing halohydrin dehalogenase was limited by product inhibition. To solve this problem and improve the ECH yield, a biotransformation strategy using resin-based in situ product removal (ISPR) was investigated. Seven macroporous resins were examined to adsorb ECH: resin HZD-9 was the best. When 10 % (w/v) HZD-9 was added to batch biotransformation, 53.3 mM ECH was obtained with a molar yield of 88.3 %. The supplement of the HZD-9 increased the ECH volumetric productivity from 0.5 to 2.8 mmol/l min compared to without addition of resin. In fed-batch biotransformation, this approach increased ECH from 31 to 87 mM. These results provide a promising basis for the biosynthesis of ECH.

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Acknowledgments

This research was partially funded by National Natural Science Foundation of China (No. 21176224), Key Project for Natural Science Foundation of Zhejiang Province (No. Z4080032), Scientific Research Project of Education Department of Zhejiang Province (No. Y201121607) and Research Fund for the Doctoral Program of Higher Education of China (New Teachers) (No. 2012331712004).

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  1. Institute of Bioengineering, Zhejiang University of Technology, Hangzhou, 310014, People’s Republic of China

    Shu-Ping Zou, Er-Hong Du, Zhong-Ce Hu & Yu-Guo Zheng

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  1. Shu-Ping Zou
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  2. Er-Hong Du
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  3. Zhong-Ce Hu
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  4. Yu-Guo Zheng
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Correspondence to Yu-Guo Zheng.

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Zou, SP., Du, EH., Hu, ZC. et al. Enhanced biotransformation of 1,3-dichloro-2-propanol to epichlorohydrin via resin-based in situ product removal process. Biotechnol Lett 35, 937–942 (2013). https://doi.org/10.1007/s10529-013-1165-y

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  • DOI: https://doi.org/10.1007/s10529-013-1165-y

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