Abstract
High-level constitutive gene expression can result in cellular metabolic imbalance and limit production. To circumvent these problems, a P alsSD -controlled auto-inducible 2-ketoisovalerate biosynthetic pathway and a P spac -controlled IPTG-inducible Ehrlich pathway were constructed in Bacillus subtilis to modulate gene expression. Based on the precise gene expression characteristics of the two inducible pathways, the optimal IPTG induction time point and dose for 2-methyl-1-propanol biosynthesis were determined as 9.5 h and 300 μM, respectively. Under the optimized conditions, strain BSUΔL-03 with inducible pathways produced up to 3.83 ± 0.46 g 2-methyl-1-propanol/l, which was about 60 % higher than BSUL04 with constitutive pathways.
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Acknowledgment
The authors appreciate the kind donation of plasmid pMUTIN4 from Dr. Danier R. Zeigler and the Bacillus Genetic Stock Center (BGSC), The Ohio State University. This research was financially supported by the National 973 Project of China (No. 2007CB714302), the Key Program of National Natural Science Foundation of China (Grant No. 20936002), National Natural Science Foundation of China (No. 20976124), and Specialized Research Fund for the Doctoral Program of Higher Education (20110032130005).
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Li, S., Jia, X. & Wen, J. Improved 2-methyl-1-propanol production in an engineered Bacillus subtilis by constructing inducible pathways. Biotechnol Lett 34, 2253–2258 (2012). https://doi.org/10.1007/s10529-012-1041-1
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DOI: https://doi.org/10.1007/s10529-012-1041-1