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Low-intensity pulsed ultrasound-mediated stimulation of hematopoietic stem/progenitor cell viability, proliferation and differentiation in vitro

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Abstract

Low-intensity pulsed ultrasound (LIPUS) stimulated the viability, proliferation and differentiation of hematopoietic stem/progenitor cells (HSPC) from fresh and cryopreserved peripheral blood leukapheresis product, as well as cord blood when applied for 10 min each day for 4 days. Cell viability, proliferation and differentiation were assessed on day 5 by viable cell counting, MTS proliferation assay, flow cytometry, and colony-forming unit assay. LIPUS stimulation: (i) enhanced the proliferation of fresh HSPC and maintained the viability of cryopreserved HSPC in vitro; (ii) did not affect the percentage of CD34+ and CD14+ cells; and (iii) enhanced burst-forming unit-erythroid colony formation. Hence, we suggest that this novel LIPUS stimulation approach might enhance the efficacy of clinical transplantation and cellular therapies using HSPC.

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Acknowledgments

The authors would like to acknowledge funding support from the Canadian Institutes of Health Research (CIHR), Natural Sciences and Engineering Research Council of Canada, the Industrial Research Assistance Program of the National Research Council of Canada, and Canadian Blood Services (CBS)/CIHR Blood Utilization and Conservation Initiative (XE00025 to AJW). The authors would also like to acknowledge the valuable discussions and inputs of Dr. Tarek El-Bialy (Department of Dentistry, the University of Alberta, Edmonton, Alberta, Canada). We are grateful for the frozen leukapheresis products provided by Brenda Letcher, and for technical assistance of Maria Cabuhat (Stem Cell Group, CBS, Edmonton, Alberta, Canada). We thank Donna Dawson (Perinatal Research Centre, University of Alberta) for the fresh cord blood collections.

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Authors and Affiliations

  1. ECERF W6-019, Department of Biomedical Engineering, University of Alberta, Edmonton, AB, T6G 2V4, Canada

    Peng Xu, Hilal Gul-Uludag, Xiaoyan Yang, Min Huang & Jie Chen

  2. National Institute for Nanotechnology, Edmonton, AB, T6G 2M9, Canada

    Peng Xu & Jie Chen

  3. Department of Electrical and Computer Engineering, University of Alberta, Edmonton, AB, T6G 2V4, Canada

    Hilal Gul-Uludag & Jie Chen

  4. IntelligentNano Inc., Edmonton, AB, T6G 2M9, Canada

    Hilal Gul-Uludag, Woon T. Ang & James Xing

  5. Canadian Blood Services R&D, Edmonton, AB, T6G 2R8, Canada

    Leah Marquez-Curtis, Locksley McGann & Anna Janowska-Wieczorek

  6. Department of Medicine, University of Alberta, Edmonton, AB, T6G 2R8, Canada

    Anna Janowska-Wieczorek

  7. Cross Cancer Institute, Edmonton, AB, T6G 1Z2, Canada

    James Xing

  8. National Research Council of Canada/IRAP, Edmonton, AB, T6N 1E4, Canada

    Eric Swanson

Authors
  1. Peng Xu
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  2. Hilal Gul-Uludag
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  3. Woon T. Ang
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  4. Xiaoyan Yang
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  5. Min Huang
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  6. Leah Marquez-Curtis
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  7. Locksley McGann
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  8. Anna Janowska-Wieczorek
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  9. James Xing
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  10. Eric Swanson
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  11. Jie Chen
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Corresponding author

Correspondence to Jie Chen.

Additional information

Peng Xu and Hilal Gul-Uludag contributed equally to this study.

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Xu, P., Gul-Uludag, H., Ang, W.T. et al. Low-intensity pulsed ultrasound-mediated stimulation of hematopoietic stem/progenitor cell viability, proliferation and differentiation in vitro. Biotechnol Lett 34, 1965–1973 (2012). https://doi.org/10.1007/s10529-012-0984-6

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  • DOI: https://doi.org/10.1007/s10529-012-0984-6

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